一例医务人员羊布鲁菌血流感染的实验室诊断及思考  

Laboratory diagnosis and reflection on a case of Brucella lamblia bloodstream infection among medical staff

作　　者：向韬 陈莎娜 XIANG Tao;CHEN Sha-Na(Department of Laboratory Medicine,Chonggang General Hospital,Chongqing 400081,China)

机构地区：[1]重钢总医院检验科,重庆400081

出　　处：《实验室检测》2024年第12期97-99,共3页Laboratory Testing

摘　　要：目的 研究实验室布鲁菌检测的方法并进行临床效果评价。方法 以我院2019年3月医务人员确诊为羊布鲁菌感染的临床病例为研究对象,通过分析与探讨血清凝集试验、常规血培养及细菌鉴定、质谱鉴定方法在诊断布鲁菌的优缺点,为基层医院实验室布鲁菌的检测工作提供一些参考。结果 该医务人员的血清布病虎红平板凝集抗原实验阳性;血培养美国BD PhoenixTM全细菌鉴定/药敏系统仪器提示需氧瓶报阳,但不能够鉴定出布鲁菌的种属;质谱仪鉴定为羊布鲁氏菌。结论 介于以上方法应用各有优缺点,基层医院实验室应根据实际情况选择适合的方法检测布鲁菌。Objective To study the method of laboratory Brucella detection and evaluate its clinical efficacy.Methods The clinical case of Brucella infection in sheep diagnosed by medical staff in our hospital in March 2019 was taken as the research object.The advantages and disadvantages of serum agglutination test,routine blood culture and bacterial identification,and mass spectrometry identification methods in diagnosing Brucella were analyzed and discussed,providing some reference for the detection of Brucella in grassroots hospital laboratories.Results The medical staff’s serum brucellosis tiger red plate agglutination antigen test was positive.The blood culture BD PhoenixTM whole bacterial identification/drug sensitivity system instrument in the United States indicates that the aerobic bottle reports positive,but cannot identify the species of Brucella.The mass spectrometer identified it as Brucella lamblia.Conclusion The above methods have their own advantages and disadvantages in application,grassroots hospital laboratories should choose suitable methods for detecting Brucella based on actual conditions.

关 键 词：布鲁菌检测 血清凝集试验 血培养 细菌鉴定 质谱鉴定 

分 类 号：R446.5[医药卫生—诊断学] R516.7[医药卫生—临床医学]