DPPH法评价黄精及炮制品抗氧化活性方法的研究  

Study on DPPH Method for Evaluating Antioxidant Activity of Huangjing(Rhizoma Polygonati)and Its Processed Products

作　　者：戴万生 杨丽 邱斌 贺森 顾雯 DAI Wansheng;YANG Li;QIU Bin;HE Sen;GU Wen(Yunnan University of Chinese Medicine,Kunming 650500,Yunnan,China)

机构地区：[1]云南中医药大学,云南昆明650500

出　　处：《辽宁中医杂志》2024年第5期152-154,223,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：云南省科技厅-云南中医药大学应用基础研究联合专项项目(2017FF116-017);云南省生物医药重大专项项目(202102AA310045)

摘　　要：目的 建立DPPH法(1,1-二苯基-2-三硝基苯肼)黄精及炮制品抗氧化活性评价方法。方法 采用超声提取和溶剂萃取提取滇黄精及其炮制品中乙醇提取物、粗皂苷、低聚糖、粗多糖4种有效组分,考察确定DPPH法测定滇黄精抗氧化活性的最佳条件,以维生素C为阳性对照,测定比较滇黄精有效组分的抗氧化能力。结果 DPPH初始浓度为0.08 mg/mL,波长为523 nm,反应时间为60 min(或180 min)为最佳条件。滇黄精炮制后对DPPH自由基的清除率提高,各有效组分对DPPH自由基的清除率为:维生素C>粗皂苷>乙醇提取物>低聚糖>粗多糖。结论 建立的DPPH法抗氧化活性检测方法灵敏、简便、快捷,可用于滇黄精及其炮制品的质量评价。Objective To establish the evaluation method on antioxidant activity of Huangjing(Rhizoma Polygonati)and its processed products by DPPH.Methods The ethanol extract,crude saponin,oligosaccharide and crude polysaccharide from Huangjing(Rhizoma Polygonati)and its processed products were extracted by ultrasonic extraction and solvent extraction.The best conditions for determining the antioxidant activity of Huangjing(Rhizoma Polygonati)by DPPH method were investigated.The antioxidant activity of the effective components of Huangjing(Rhizoma Polygonati)was determined and compared with vitamin C as the positive control.Results The initial concentration of DPPH was 0.08 mg·L-1.The wavelength was 523 nm,and the reaction time was 60 min(or 180 min).The scavenging rate of DPPH free radicals was improved after processing of Huangjing(Rhizoma Polygonati).The scavenging rate of DPPH free radicals by each effective component was vitamin C>crude saponin>ethanol extract>oligosaccharide>crude polysaccharide.Conclusion The established DPPH method is sensitive,simple and rapid,and can be used to evaluate the quality of Huangjing(Rhizoma Polygonati)and its processed products.

关 键 词：滇黄精 抗氧化活性 DPPH 总皂苷 乙醇提取物 

分 类 号：R283[医药卫生—中药学]