一株好氧反硝化细菌HL3的鉴定及脱氮性能研究  

Identification and Nitrogen Removal Performance of an Aerobic Denitrifying Bacterium HL3

作　　者：刘凤坤 赵吉臣 许敬轩 阮灼豪 杨岚心 郭慧[1] 黄文 LIU Fengkun;ZHAO Jichen;XU Jingxuan;RUAN Zhuohao;YANG Lanxin;GUO Hui;HUANG Wen(College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangdong Key Laboratory of Animal Breeding and Nutrition,South China Key Laboratory of Animal Nutrition and Feed,Ministry of Agriculture and Rural Areas,Aquatic Research Center,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;School of Aquaculture,Tianjin Agricultural University,Tianjin 300300,China;Heyuan Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Heyuan 517000,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]广东省农业科学院动物科学研究所,广东省畜禽育种与营养研究重点实验室,农业农村部华南动物营养与饲料重点实验室,广东省农业科学院水产研究中心,广东广州510640 [3]天津农学院水产学院,天津300300 [4]岭南现代农业科学与技术广东省实验室河源分中心,广东河源517000

出　　处：《环境科学与技术》2024年第S2期123-132,共10页Environmental Science & Technology

基　　金：广东省农业科学院人才项目(R2020YJ-QG001,R2022PY-QY008);珠海市斗门区白蕉镇政府服务项目(ZHWZ2021-403FW,ZHWZ2021-408FW);岭南现代农业科学与技术广东省实验室河源分中心课题(DT20220019);广东省农业科学院协同创新中心课题(202137,202138,XT202231);广东省农业科学院人才项目(R2020YJ-QG001,R2022PY-QY008)

摘　　要：从海鲈养殖池塘底泥中分离得到了1株好氧反硝化细菌HL3,通过对HL3进行形态学、生理生化以及16S r DNA序列鉴定,确定HL3为嗜线虫沙雷氏菌(Serratia nematodiphila)。从碳源、C/N、p H和温度4个环境因素对HL3生长和脱氮性能进行研究,结果表明,当以琥珀酸钠为碳源、C/N=10、p H=7和温度为30℃时,HL3有最佳脱氮能力,48 h内TN、NH4_(+)^(-)N、NO_(2)^(-)-N、NO_(3)^(-)-N去除率分别为78.23%、92.80%、99.57%和94.65%。好氧反硝化细菌Serratia nematodiphila HL3有较高的生物安全性和脱氮效果,该菌在养殖尾水的生物脱氮处理方向有广阔的应用前景。An aerobic denitrifying bacterium HL3 was isolated from the bottom mud of the sea bass culture pond.Through morphological,physiological and biochemical identification of HL3 and 16S rDNA sequence,HL3 was identified as Serratia nematodiphila.The growth and denitrification performance of HL3 were studied from four environmental factors:carbon source,C/N,pH and temperature.The results showed that when sodium succinate was used as carbon source,C/N=10,pH=7 and temperature was 30℃,HL3 had the best denitrification ability.Within 48 h,the removal rates of TN、NH4_(+)^(-)N、NO_(2)^(-)-N and NO_(3)^(-)-N were 78.23%,92.80%,99.57%and 94.65%,respectively.The aerobic denitrifying bacteria Serratia nematodiphila HL3 has high biosafety and nitrogen removal effect,and it has broad application prospects in the biological nitrogen removal treatment direction of aquaculture tail water.

关 键 词：好氧反硝化细菌Serratia nematodiphila HL3 生物安全性 环境因素 脱氮能力 

分 类 号：X172[环境科学与工程—环境科学]