微重力对休眠成肌细胞的影响  

The effect of microgravity on hibernating myoblasts

作　　者：刘一洲 曹晓健 施镠佳 陈云强 谭映军[3] 黄丹霞 王春艳[3] 周秋至 曲丽娜[3] 罗红梅 殷学民[3] 张松[1] 刘朝霞[3] 李雅洁 徐佳 李莹辉[3] 陈红[1,2] Liu Yizhou;Cao Xiaojian;Shi Liujia;Chen Yunqiang;Tan Yingjun;Huang Danxia;Wang Chunyan;Zhou Qiuzhi;Qu Lina;Luo Hongmei;Yin Xuemin;Zhang Song;Liu Zhaoxia;Li Yajie;Xu Jia;Li Yinghui;Chen Hong(Department of Rehabilitation,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430000,China;Stem Cell Research Center,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430000,China;State Key Laboratory of Space Medicine,China Astronaut Research and Training Center,Beijing 100094,China)

机构地区：[1]华中科技大学同济医学院附属同济医院康复医学科,武汉430000 [2]华中科技大学大学同济医学院附属同济医院干细胞研究中心,武汉430000 [3]中国航天员科研训练中心航天医学全国重点实验室,北京100094

出　　处：《航天医学与医学工程》2024年第5期275-281,共7页Space Medicine & Medical Engineering

基　　金：载人航天工程航天医学实验领域项目资助(HYZHXM01022)。

摘　　要：目的研究微重力环境对休眠成肌细胞的影响。方法利用真实微重力及模拟微重力培养成肌细胞10 d后,通过RNA-seq检测及免疫荧光研究微重力环境对成肌细胞基因表达及细胞生长的影响。结果休眠成肌细胞在微重力环境中与增殖相关基因表达上调,相对于正常重力组,模拟微重力组细胞数量更多且Ki67阳性率更高。结论微重力可以促进休眠成肌细胞的增殖。Objective To investigate the effects of microgravity environment on hibernating myoblasts.Methods Hibernating myoblasts were cultured under real and simulated microgravity conditions for 10 days.RNA-seq analysis and immunofluorescence are used to analysis the impact of microgravity environment on cell growth and gene expression of myoblasts.Results Under the microgravity conditions,genes associated with proliferation were upregulated.Under simulated microgravity,there were more and higher proportion of Ki67 positive cells compared to normal gravity conditions.Conclusion The microgravity environment promotes the proliferation of hibernating myoblasts.

关 键 词：休眠状态 成肌细胞 微重力 太空飞行 骨骼肌再生 

分 类 号：R73[医药卫生—肿瘤]