覃怀1号鲜地黄化学成分及其降糖活性研究  

Chemical consitituents and hypoglycemic activity of Qinhuai No.1 Rehmannia glutinosa

作　　者：杨梦[1,2] 郝志友 王小兰[1,3] 肖超远 张俊阳[1,2] 周诗琪 郑晓珂 冯卫生 YANG Meng;HAO Zhi-you;WANG Xiao-lan;XIAO Chao-yuan;ZHANG Jun-yang;ZHOU Shi-qi;ZHENG Xiao-ke;FENG Wei-sheng(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;The Engineering and Technology Research Center for Chinese Medicine Development of Henan Province,Zhengzhou 450046,China;Academy of Chinese Medical Sciences,Henan University of Chinese Medicine,Zhengzhou 450046,China;Co-construction of Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan&Education Ministry,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南省中药开发工程技术研究中心,河南郑州450046 [3]河南中医药大学中医科学院,河南郑州450046 [4]呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046

出　　处：《药学学报》2025年第1期205-210,共6页Acta Pharmaceutica Sinica

基　　金：国家重点研发计划(2017YFC1702800);河南省高层次人才特殊支持计划(ZYQR201810080)。

摘　　要：采用硅胶、MCI gel CHP-20、ODS、Toyopearl HW-40C等柱色谱技术,并结合TLC和半制备液相等手段对覃怀1号鲜地黄70%丙酮提取物的乙酸乙酯部位进行分离纯化,得到8个单体化合物。运用现代波谱学技术(NMR、MS、UV、IR)鉴定化合物的结构,分别鉴定为地黄新苷A(1)、osmanthuside B_(6)(2)、地黄苷(3)、异马蒂罗苷(4)、反式对羟基肉桂酸(5)、咖啡酸(6)、阿魏酸(7)、咖啡酸甲酯(8)。其中,化合物1为新苯乙醇苷,鉴定为地黄新苷A,化合物2为首次从地黄中分离得到。药理活性研究表明,化合物2、6和7可以显著增加相对葡萄糖消耗量,表现出潜在的降糖活性。Eight compounds were isolated and purified from the ethyl acetate part of 70%acetone extract of Rehmannia glutinosa by various chromatographic techniques such as silica gel,MCI gel CHP-20,ODS,Toyopearl HW-40C,combined with TLC and semi-preparative HPLC.Their structures were elucidated by modern spectroscopy techniques(NMR,MS,UV,IR),and identified as neomartynoside A(I),osmanthuside B,(2),martynoside(3),isomartynoside(4),(E)-p-hydroxycinnamic acid(5),caffeic acid(6),ferulic acid(7),and methyl caffeate(8).Compound 1 is a new phenylethanol glycoside,which was identified as neomartynoside A.Compound 2 was isolated from Rehmannia glutinosa for the first time.In addition,compounds 2,6 and 7 significantly increased relative glucose consumption,showing potential hypoglycemic activity.

关 键 词：地黄 地黄新苷A 苯乙醇苷 苯丙素 分离鉴定 降糖活性 

分 类 号：R284[医药卫生—中药学]