蜜环菌侵染猪苓菌核多糖与甾体类成分积累的规律研究  

Study on accumulation of polysaccharide and steroid components in Polyporus umbellatus infected by Armillaria spp.

作　　者：杨明姝 尹一飞 陈娟[1,2] 李兵 侯梦妍[1] 冷春燕 邢咏梅 郭顺星[1] YANG Ming-shu;YIN Yi-fei;CHEN Juan;LI Bing;HOU Meng-yan;LENG Chun-yan;XING Yong-mei;GUO Shun-xing(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China;State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,Beijing 100700,China)

机构地区：[1]中国医学科学院、北京协和医学院药用植物研究所,北京100193 [2]道地药材品质保障与资源持续利用全国重点实验室,北京100700

出　　处：《药学学报》2025年第1期232-238,共7页Acta Pharmaceutica Sinica

基　　金：中国医学科学院医学与健康科技创新工程项目(CIFMS)(2021-I2M-1-032);国家自然科学基金(81973425)。

摘　　要：为了探讨两种蜜环菌(Armillaria gallica和A.mellea)侵染猪苓菌核后对其不同部位的甾体类与多糖含量的影响,本研究通过检测不同采收年限猪苓菌核生物量以及菌核中侵染部位(隔离腔壁)与非侵染部位的麦角甾醇、猪苓酮A、猪苓酮B与多糖4种有效成分的含量,根据生物量与含量的差异和动态变化筛选优质蜜环菌,获得猪苓的最佳采收年限。采用高效液相色谱法与紫外-可见分光光度法分别测定猪苓菌核中上述4种成分的含量。另外,对蜜环菌侵染猪苓菌核不同部位多糖合成相关的差异基因进行筛选与分析。研究发现,不同种类蜜环菌侵染的猪苓菌核随栽培年份的增长,猪苓菌核生物量与4种成分的含量均存在显著差异,猪苓隔离腔壁部位的4种成分显著高于其非侵染部位,最佳采收年限是在栽培后的第3年。转录组学分析表明蜜环菌的侵染能够显著促进猪苓多糖合成相关差异基因高表达。本研究明确了蜜环菌侵染对猪苓菌核化学成分积累的影响,为探索两者共生机制奠定了基础,为筛选优质蜜环菌并指导猪苓人工栽培提供了科学依据。In view of the few studies on the influence of Armillaria spp.infection on the content of the chemical components in different parts of Polyporus umbellatus sclerotia,this study determined the biomass of P.umbellatus sclerotia and the contents of ergosterol,polyporusterone A,polyporusterone B and polysaccharide in the separated cavity wall of the sclerotia and the uninfected part of the sclerotia in different harvesting years under the conditions of A.gallica and A.mellea infection respectively.According to the difference of content and dynamic changes of the polysaccharide and the steroid substances,the superior Armillaria sp.was screened to obtain the best harvest years of P.umbellatus.Using HPLC and UV-VIS spectrophotometry methods,the contents of ergosterol,polyporusterone A,polyporusterone B and polysaccharide in P.umbellatus sclerotia infected by the two Armillaria spp.in different years were determined.In addition,the differentially expressed genes related to P.umbellatus polysaccharide synthesis were screened according to the transcriptomic data of different parts of P.umbellatus after A.mellea infection.With the increase of years,the biomass of sclerotia infected by different Armillaria spp.had significant differences,and there were significant differences in the four components of sclerotia.The four components of the separated cavity wall of the sclerotia were significantly higher than those of the uninfected part.The best harvest time was the third year after cultivation.Transcriptomic analysis showed that the infection of Armillaria spp.could significantly promote polysaccharide synthesis,which provided a basis for polysaccharide content determination at the molecular level.The study clarified the influence of different Armillaria spp.infection on the accumulation of chemical components of P.umbellatus sclerotia,laying a foundation for exploring the symbiosis mechanism and provided a scientific clue for screening superior Armillaria sp.and guiding the artificial cultivation of P.umbellatus sclerotia.

关 键 词：猪苓 蜜环菌 麦角甾醇 猪苓酮A 猪苓酮B 多糖 转录组 

分 类 号：R931[医药卫生—生药学]