高温胁迫对克氏原螯虾肠道组织形态与转录组的影响  

Effect of high temperature stress on intestinal tissues morphology and transcriptome of Procambarus clarkii

作　　者：包志明 邹永烽 曹攀辉 张嘉媛 徐宇[2] 许志强[2] 郭慧[1] BAO Zhiming;ZOU Yongfeng;CAO Panhui;ZHANG Jiayuan;XU Yu;XU Zhiqiang;GUO Hui(College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Freshwater Fisheries Research Institute of Jiangsu Province/Key Laboratory of Genetic Breeding and Cultivation for Freshwater Crustacean,Ministry of Agriculture and Rural Affairs,Nanjing 210017,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]江苏省淡水水产研究所/农业农村部淡水虾蟹遗传育种与养殖重点实验室,江苏南京210017

出　　处：《南方水产科学》2025年第1期105-117,共13页South China Fisheries Science

基　　金：江苏种业振兴“揭榜挂帅”项目[JBGS(2021)119];广东海洋大学创新创业训练计划项目资助(CXXL2023011)。

摘　　要：为探究克氏原螯虾(Procambarus clarkii)肠道在接近其生理极限的高温胁迫下的作用机制,对其进行32和37℃的高温胁迫实验,分别于胁迫24、72 h后收集肠道组织,进行组织切片观察和转录组学分析。结果表明,高温对克氏原螯虾的肠道组织结构造成了明显损伤,且随着胁迫温度和胁迫时间的增加,损伤加剧。与对照组(26℃)相比,在32℃胁迫24和72 h条件下,分别鉴定到2 462和4 619个差异基因;在37℃胁迫24和72 h条件下,分别鉴定到1 825和7 298个差异基因。KEGG富集分析结果显示,差异基因在内质网蛋白加工、代谢途径等通路中显著富集,其中在同一胁迫时间内胁迫温度从32℃升至37℃时,内质网蛋白加工通路富集频率升高,表明其在应对高温胁迫中发挥重要作用;GO功能注释显示,差异基因主要在碳水化合物代谢、蛋白质折叠等相关过程中显著富集。To explore the mechanism of the intestinal tract of red swamp crayfish(Procambarus clarkii)under high tempera-ture stress close to its physiological limit,we conducted the high-temperature stress experiments at 32℃and 37℃,and collec-ted the intestinal tissues after 24 h and 72 h of stress,for tissue sectioning observation and transcriptomic analysis.The results show that high temperature caused significant damages to the intestinal tissue structure of crayfish,and the damage increased with the increase of stress temperature and stress time.Compared with the control group(26℃),2462 and 4619 differentially expressed genes(DEGs)were identified during 32℃stress for 24 h and 72 h,respectively.During 37℃stress for 24 h and 72 h,1825 and 7298 DEGs were identified,respectively.KEGG enrichment analysis shows that the DEGs were significantly enriched in the endoplasmic reticulum protein processing and metabolic pathways,and the enrichment frequency of endoplasmic reti-culum protein processing pathways increased when the stress temperature increased from 32℃to 37℃during the same stress time,which indicates that they played an important role in coping with high temperature stress.According to the GO functional annota-tion,DEGs were mainly enriched in the energy metabolism-related processes such as carbohydrate metabolism and protein folding.

关 键 词：克氏原螯虾 高温胁迫 转录组学 肠道 石蜡切片 

分 类 号：S917.4[农业科学—水产科学]