乳酸通过DJ-1增强HT22细胞低氧耐受能力  

Lactate enhances hypoxia tolerance in HT22 cells via DJ-1

作　　者：蔡珂沁 吕军[1] 李文欣 石瑞丽[1] 马宝慧[1] 郝肖琼 时静华[1] 齐瑞芳[1,2] CAI Keqin;LYU Jun;LI Wenxin;SHI Ruili;MA Baohui;HAO Xiaoqiong;SHI Jinghua;QI Ruifang(School of Basic Medicine and Forensic Sciences,Baotou Medical College of Inner Mongolia University of Science and Technology,Baotou 014040,China;Inner Mongolia Key Laboratory of Hypoxia of the Center for Translational Medicine,Baotou Medical College of Inner Mongolia University of Science and Technology)

机构地区：[1]内蒙古科技大学包头医学院基础医学与法医学院,内蒙古包头014040 [2]内蒙古转化医学中心低氧重点实验室

出　　处：《包头医学院学报》2025年第1期9-13,共5页Journal of Baotou Medical College

基　　金：国家自然科学基金资助项目(No 82360272);包头医学院科学研究基金项目(No BYJJ-BSJJ-202003);大学生“花蕾计划”项目(110-2023300734)。

摘　　要：目的:探究在低氧条件下乳酸对小鼠海马HT22细胞的神经保护作用。方法:将HT22细胞分成4组:对照组、乳酸组(LA组)、低氧组(Hypoxia组)和低氧加乳酸组(Hypoxia+LA组),通过CCK-8检测低氧条件下不同浓度乳酸对细胞活力的影响,观察细胞形态的变化;采用Western blot检测由PARK7(Parkinson's disease-associated protein 7, PARK7)基因编码的DJ-1的表达,并通过细胞免疫荧光法检测DJ-1分布和表达变化。结果:与低氧组相比,1∶10^(4)的乳酸的添加明显提高了细胞活力,具有统计学意义(P<0.01);与对照组相比,低氧后DJ-1蛋白在细胞内分布与表达减少,具有统计学意义(P<0.01);与低氧组相比,在低氧前加入乳酸增加了细胞中DJ-1的表达与分布,具有统计学意义(P<0.05)。结论:低氧前乳酸加入可能通过激活DJ-1,维持细胞活性,缓解低氧损伤,从而发挥神经保护作用。Objective:To investigate the neuroprotective effect of lactic acid on mouse hippocampal HT22 cells under hypoxic conditions.Methods:HT22 cells were divided into four groups:the control group,lactate group(LA group),hypoxia group and hypoxia+lactic acid group(Hypoxia+LA group),and CCK-8 was used to detect the effects of different concentrations of lactate on cell viability under hypoxic conditions,and the effects of morphological changes were observed.The expression of DJ-1(Parkinson's disease-associated protein 7)encoded by PARK7 gene was detected by Western blot,and changes in DJ-1 distribution and expression were detected by cellular immunofluorescence.Results:Compared with the hypoxia group,the addition of 1∶10^(4) lactic acid significantly increased cell viability,which was statistically significant(P<0.01).Compared with the normal control group,the intracellular distribution and expression of DJ-1 protein decreased under hypoxia condition,which was statistically significant(P<0.01).The addition of lactic acid before hypoxia increased the expression and distribution of DJ-1 in the cells compared with the hypoxia group,which was statistically significant(P<0.05).Conclusion:Pre-hypoxic lactate incorporation may exert neuroprotective effects by activating DJ-1,maintaining cellular activity,and mitigating hypoxic injury.

关 键 词：低氧 乳酸 DJ-1 抗氧化 HT22 神经保护 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]