大鼠脑缺血再灌注损伤后神经功能及行为学改变  

Changes of neurological function and behavior of rats after cerebral ischemia-reperfusion injury

作　　者：王璐 张士滨 吕雪[1] 贾建新[2] 杨占君[2] 吴丽娥[3] WANG Lu;ZHANG Shibin;LV Xue;JIA Jianxin;YANG Zhanjun;WU Li′e(Graduate School,Baotou Medical College,Inner Mongolia,Baotou 014040,China;Department of Human Anatomy,Baotou Medical College;The First Affiliated Hospital,Baotou Medical College)

机构地区：[1]包头医学院研究生学院,内蒙古包头014040 [2]包头医学院人体解剖学教研室 [3]包头医学院第一附属医院

出　　处：《包头医学院学报》2025年第1期20-24,59,共6页Journal of Baotou Medical College

基　　金：国家自然科学基金项目(81860215);内蒙古自然科学基金项目(2021MS08010);内蒙古自治区高等学校“青年科技英才支持计划”(NJYT-20-A08);内蒙古自治区高等学校创新团队发展计划(NMGIRT2328)。

摘　　要：目的:探究大鼠脑缺血再灌注损伤后神经功能缺损情况及行为学的变化。方法:将60只雄性Wistar大鼠随机分为Sham组、24 h组、48 h组、72 h组、7 d组、14 d组,采用大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)法构建大鼠局灶性脑缺血再灌注损伤(cerebral ischemia reperfusion injury,CIRI)模型。通过改良大鼠神经功能缺损评分(modified neurological severity score,mNSS)评估各组大鼠神经功能缺损情况,监测大鼠体重并记录其变化;TTC染色检测各组大鼠梗死面积;贴纸去除实验、旷场实验、转棒实验评估各组大鼠感觉和运动能力、情绪变化情况。结果:大鼠脑缺血再灌注后体重明显降低(P<0.01),梗死面积增加(P<0.01),以72 h最为显著;与Sham组比较,脑缺血再灌注后大鼠神经功能缺损评分升高(P<0.01),贴纸去除时间增加(P<0.01),并在48~72 h达高峰。旷场实验结果表明MCAO模型大鼠运动路程减少(P<0.01),静止时间增加(P<0.01),模型大鼠平均速度和站立次数均减少(P<0.01),出现了抑郁情绪,对外界的好奇心和探索欲望减退。转棒实验结果显示MCAO模型大鼠运动时间和运动距离减少(P<0.01),且均在48~72 h变化更为显著。结论:脑缺血再灌注损伤48~72 h对大鼠神经功能、运动能力减弱,肢体协调性及情绪影响最为严重。Objective:To explore the neurological deficit and behavioral changes of rats after cerebral ischemia-reperfusion injury.Methods:A total of 60 male Wistar rats were randomly divided into the Sham group,24 h group,48 h group,72 h group,7 d group and 14 d group,and the rat model of focal cerebral ischemia reperfusion injury(CIRI)was established using middle cerebral artery occlusion(MCAO).The neurological deficit of rats in each group was evaluated by modified neurological severity score(mNSS),and the weight of rats was monitored and recorded.The rate of cerebral infarction was detected by TTC staining,the sensory and motor abilities and emotional changes of rats in each group were evaluated by Adhesive Removal Test,Open Field Test and Rotarod Test.Results:After cerebral ischemia/reperfusion,the weight of rats decreased significantly(P<0.01),especially at 72 h;Compared with the Sham group,the neurological deficit score of rats after cerebral ischemia-reperfusion increased significantly(P<0.01),and the removal time of adhesive tape increased significantly(P<0.01),reaching the peak at 48~72 h.The results of open field test showed that the moving distance of rats of the MCAO model was significantly decreased(P<0.01),while the rest time was significantly increased(P<0.01),with the significantly decreased average speed and standing time(P<0.01),and rats of MCAO model were depressed,with the lowered curiosity and desire to explore the outside world.The results of rotarod test showed that the moving time and distance of rats in the MCAO model were decreased(P<0.01),and the changes were more significant from 48~72 h.Conclusion:Cerebral ischemia-reperfusion injury after 48~72 h could weaken the neurological function and motor ability of rats,which seriously affect limb coordination and emotion of rats.

关 键 词：脑缺血再灌注 大脑中动脉闭塞 神经功能缺损 行为学 

分 类 号：R743.3[医药卫生—神经病学与精神病学]