LAT1、LC3Ⅱ及TSHZ3水平对膀胱癌诊断和手术后复发风险评估分析  

Analysis of LAT1,LC3 Ⅱ and TSHZ3 levels in diagnosis and postoperative recurrence risk assessment of bladder cancer

作　　者：曹建伟[1] 穆一姝 付国 赵华才[1] 高丹 CAO Jianwei;MU Yishu;FU Guo;ZHAO Huacai;GAO Dan(Department of Urology,Shanxi Provincial People's Hospital,Xian 710068,China)

机构地区：[1]陕西省人民医院泌尿外科,陕西西安710068

出　　处：《西部医学》2025年第2期256-261,共6页Medical Journal of West China

基　　金：陕西省自然科学基础研究计划项目(2021JQ-908)。

摘　　要：目的 探讨L型氨基酸转运蛋白1(LAT1)、微管相关蛋白1轻链3Ⅱ(LC3Ⅱ)及锌指同源盒3(TSHZ3)水平对膀胱癌诊断和手术后复发风险评估意义。方法 选取2019年9月—2022年9月在我院行尿道膀胱肿瘤电切术的膀胱癌患者96例癌组织与癌旁组织。所有患者均随访12个月,记录患者术后复发情况,分为复发组及无复发组。采用免疫组织化学法测定LAT1、LC3Ⅱ及TSHZ3蛋白阳性表达。比较癌组织与癌旁组织LAT1、LC3Ⅱ及TSHZ3蛋白阳性表达;比较不同病理特征LAT1、LC3Ⅱ及TSHZ3蛋白阳性表达;比较复发组与无复发组蛋白LAT1、LC3Ⅱ及TSHZ3阳性表达。采用ROC曲线分析LAT1、LC3Ⅱ及TSHZ3表达对膀胱癌及术后复发预测价值。采用多因素Logistic回归分析LAT1、LC3Ⅱ及TSHZ3表达与术后复发关系。结果 癌组织LAT1蛋白阳性表达率高于癌旁组织,而LC3Ⅱ及TSHZ3蛋白阳性表达率低于癌旁组织(均P<0.05)。不同性别、年龄和肿瘤最大径LAT1、LC3Ⅱ及TSHZ3蛋白表达比较差异均无统计学意义(P>0.05)。T1期LAT1阳性表达率高于Ta期,淋巴结转移LAT1阳性表达率高于无淋巴结转移(P<0.05);T1期LC3Ⅱ和TSHZ3阳性表达率低于Ta期,淋巴结转移LC3Ⅱ和TSHZ3阳性表达率低于无淋巴结转移(均P<0.05)。复发组LAT1阳性表达率高于无复发组,而LC3Ⅱ和TSHZ3阳性表达率低于无复发组(均P<0.05)。ROC曲线分析显示,膀胱癌预测中,LAT1灵敏度92.20%,特异度为88.90%;LC3Ⅱ灵敏度100.00%,特异度为86.70%;TSHZ3灵敏度91.10%,特异度为91.10%。ROC曲线分析显示,膀胱癌术后复发预测中,LAT1灵敏度87.50%,特异度为92.20%;LC3Ⅱ灵敏度96.90%,特异度为79.70%;TSHZ3灵敏度78.10%,特异度为95.30%。经多因素Logistic回归分析显示,LAT1高表达、LC3Ⅱ低表达及TSHZ3低表达为影响术后复发的独立危险因素(P<0.05)。结论 膀胱癌患者LAT1呈高表达而LC3Ⅱ和TSHZ3呈低表达,LAT1、LC3Ⅱ、TSHZ3与术后复发密切相关,对预测膀Objective To investigate the significance of the levels of L-amino acid transporter 1(LAT1),microtubule associated protein 1 light chain 3 Ⅱ(LC3 Ⅱ) and zinc finger homeobox 3(TSHZ3) in the diagnosis of bladder cancer and the risk assessment of postoperative recurrence.Methods 96 patients with bladder cancer who underwent transurethral resection of bladder tumors in our hospital from September 2019 to September 2022 were selected.All patients were followed up for 12 months and their postoperative recurrence was recorded.Immunohistochemical methods were used to determine the positive expression of LAT1,LC3II,and TSHZ3 proteins.Compare the positive expression of LAT1,LC3II,and TSHZ3 proteins in cancer tissues and adjacent tissues;Compare the positive expression of LAT1,LC3II,and TSHZ3 proteins in different pathological features;Compare the positive expression of LAT1,LC3II,and TSHZ3 proteins between the recurrent group and the non recurrent group.ROC curve was used to analyze the predictive value of LAT1,LC3 Ⅱ and TSHZ3 expression for bladder cancer and postoperative recurrence.Multiple logistic regression analysis was used to investigate the relationship between LAT1,LC3II,and TSHZ3 expression and postoperative recurrence.Results The cancer tissue positive expression rate of LAT1 was protein higher than adjacent tissue,while the positive expression rates of LC3II and TSHZ3 protein was lower than adjacent tissue(P<0.05).The expression of LAT1,LC3II,and TSHZ3 proteins among different genders,ages,and maximum tumor diameter had no statistically significant difference(P>0.05).The T1 phase positive expression rate of LAT1 was higher Ta phase,and the lymph node metastasis positive expression rate of LAT1 was higher than non lymph node metastasis(P<0.05).The T1 stage positive expression rates of LC3II and TSHZ3 were lower than that of Ta stage,and the lymph node metastasis positive expression rates of LC3II and TSHZ3 were lower than that of non lymph node metastasis(P<0.05).The recurrent group positive expression ra

关 键 词：L型氨基酸转运蛋白1 微管相关蛋白1轻链3Ⅱ 锌指同源框蛋白3 膀胱癌 术后复发 

分 类 号：R737.14[医药卫生—肿瘤]