MYO6和PRAS40在胃癌淋巴结转移中的表达及其意义  

The Expression and Significance of MYO6 and PRAS40 in Lymph Node Metastasis of Gastric Cancer

作　　者：郭明海 刘记恩[1] 刘小慧 张云杰[1] 王伟栋 李明辉[1] Guo Minghai;Liu Ji'en;Liu Xiaohui(Department 8 of General Surgery and Department 2 of General Surgery,Handan Central Hospital,Handan,Hebei 056002,China)

机构地区：[1]邯郸市中心医院东区普外八科,普外二科,河北邯郸056002

出　　处：《四川医学》2025年第1期1-6,共6页Sichuan Medical Journal

基　　金：河北省重点研发计划健康医疗与生物医药专项项目(编号:172777201)。

摘　　要：目的探讨肌球蛋白Ⅵ(MYO6)和富含脯氨酸的Akt底物40(PRAS40)在胃癌淋巴结转移中的表达及意义。方法回顾性选取我院2018年5月至2020年5月收治的胃癌淋巴结转移患者108例为观察组。另选取同期我院进行治疗的胃癌无淋巴结转移患者79例作为对照组。采用免疫组织化学法检测观察组胃癌淋巴结转移组织、胃癌原发组织、癌旁正常组织及对照组胃癌组织中MYO6和PRAS40的表达;胃癌淋巴结转移组织MYO6和PRAS40表达的相关性采用Spearman法分析;采用Kaplan-Meier法分析MYO6、PRAS40表达与胃癌淋巴结转移患者3年总生存率的关系;采用多因素Cox回归分析影响胃癌淋巴结转移患者预后的因素。结果观察组胃癌组织中MYO6、PRAS40的阳性率显著高于对照组(P<0.05);淋巴结转移组织中MYO6、PRAS40的阳性率显著高于胃癌原发组织中MYO6、PRAS40的阳性率(P<0.05),胃癌原发组织中MYO6、PRAS40的阳性率显著高于癌旁正常组织中MYO6、PRAS40的阳性率(P<0.05);TNM分期为Ⅲ期、浸润深度为深层、细胞分化程度为低分化的胃癌淋巴结转移患者MYO6、PRAS40阳性表达比例均高于TNM分期为Ⅱ期、浸润深度为浅层、细胞分化程度为中/高分化的胃癌淋巴结转移患者(P<0.05);胃癌淋巴结转移组织中MYO6与PRAS40表达呈正相关(r=0.437,P<0.001);MYO6、PRAS40阳性表达胃癌淋巴结转移患者3年生存率显著低于MYO6、PRAS40阴性表达患者(P<0.05);MYO6、PRAS40、TNM分期是胃癌淋巴结转移患者死亡的危险因素(P<0.05)。结论胃癌原发组织及转移淋巴结组织MYO6和PRAS40阳性表达率均升高,与胃癌淋巴结转移患者临床病理特征及预后密切相关,可能参与胃癌淋巴结转移灶的形成。Objective To investigate the expression and significance of myosinⅥ(MYO6)and proline rich Akt substrate 40(PRAS40)in lymph node metastasis of gastric cancer.Methods A total of 108 patients with lymph node metastasis of gastric cancer hospitalized in our hospital from May,2018 to May,2020 were retrospectively selected as the observation group.In addition,79 gastric cancer patients without lymph node metastasis treated in our hospital during the same period were selected as the control group.Immunohistochemistry was used to detect the expression of MYO6 and PRAS40 in lymph node metastasis tissues,primary gastric cancer tissues and adjacent normal tissues in the observation group and gastric cancer tissues in the control group;the correlation between the expression of MYO6 and PRAS40 in lymph node metastasis tissues of gastric cancer was analyzed using Spearman method;Kaplan-Meier method was used to analyze the relationship between MYO6 and PRAS40 expression and 3-year overall survival rate of gastric cancer patients with lymph node metastasis.Multivariate Cox regression analysis was used to analyze the factors affecting the prognosis of gastric cancer patients with lymph node metastasis.Results The positive rates of MYO6 and PRAS40 in gastric cancer tissues in the observation group were significantly higher than those in the control group(P<0.05);the positive rates of MYO6 and PRAS40 in lymph node metastasis tissues were obviously higher than those in primary gastric cancer tissues(P<0.05),the positive rates of MYO6 and PRAS40 in primary gastric cancer tissues were obviously higher than those in normal adjacent tissues(P<0.05);the positive expression rates of MYO6 and PRAS40 in gastric cancer lymph node metastasis patients with TNM stageⅢ,deep infiltration depth,and low cell differentiation were higher than those in gastric cancer lymph node metastasis patients with TNM stageⅡ,shallow infiltration depth,and medium/high cell differentiation degree(P<0.05);there was a positive correlation between the expression

关 键 词：肌球蛋白Ⅵ 富含脯氨酸的Akt底物40 胃癌 淋巴结转移 表达 

分 类 号：R735.2[医药卫生—肿瘤]