HS及HSP70功能抑制对MCF-7乳腺癌细胞增殖的影响  

Effects of HS and HSP70 function inhibition on the proliferation of MCF-7 breast cancer cells

作　　者：喻碧莺[1] 章小曼[1] 储许可 吴吴淑 Biying Yu;Xiaoman Zhang;Xuke Chu;Shulian Wu(Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education,Fujian Provincial Key Laboratory for Photonics Technology,College of Photonic and Electronic Engineering,Fujian Normal University,Fuzhou 350007,China)

机构地区：[1]福建师范大学医学光电科学与技术教育部重点实验室,福建省光子技术重点实验室,光电与信息工程学院,福建福州350007

出　　处：《福光技术》2024年第2期51-56,共6页FUJIAN OPTICAL TECHNOLOGY

基　　金：福建省自然科学基金面上项目(2021J011642022J01628)。

摘　　要：热休克蛋白70(Heatshock70,HSP70)与乳腺肿瘤形成、发展、治疗、预后和肿瘤治疗中形成的药物抵抗有关,从而成为抗肿瘤治疗的新靶点。VER-155008是热休克蛋白70的有效抑制剂。本文采用MTT比色法研究单独热休克(Heat Shock,HS)作用,单独VER-155008作用以及两者联合作用对MCF-7乳腺癌细胞增殖的影响。结果表明适当的HS作用时长对MCF-7乳腺癌细胞具有促增殖效应,当HS作用时间过长则具有增殖抑制效果。HSP70功能抑制导致细胞增殖抑制,其增殖抑制效果与VER-155008抑制剂作用的剂量和时长呈正相关。两者联合作用的效果由HSP70O功能抑制导致的细胞增殖抑制和由HS作用导致的促增殖或增殖抑制效应共同决定。Heat shock protein 70(HSP70)is involved with the occurrence,development,treatment,prognosis and drug resistance in breast cancer.It has become a new target for anti-cancer therapy.VER-155008 is an effective inhibitor of heat shock protein 70.The effects of Heat Shock(HS)treatment,VER-155008 treatment and their combination treatment on the proliferation of MCF-7 breast cancer cells were studied by MTT assay.The results showed that appropriate duration of HS treatment could promote the proliferation of MCF-7 breast cancer cells,while too long duration of HS treatment could inhibit the proliferation of MCF-7 breast cancer cells.The HSP70 function inhibition resulted in the inhibition of cell proliferation,and the proliferation inhibition effect was positively correlated with the dose and duration of VER-155008.The effect of the combination treatment was determined by the cell proliferation inhibition caused by theHSP70 function inhibition and the pro-proliferation or anti-proliferation effect caused by HS.

关 键 词：热休克(HS) 热休克蛋白70(HSP70) 乳腺癌细胞 细胞增殖 

分 类 号：R73[医药卫生—肿瘤]