虫类药治疗骨髓增殖性疾病的应用规律和作用机制探讨  

Study on Application Rule and Mechanism of Insect Medicinals for Myeloproliferative Diseases

作　　者：马国栋 崔思远[2] MA Guodong;CUI Siyuan(The First Clinical College of Medicine,Shandong University of Traditional Chinese Medicine,Jinan Shandong 250013,China;Department of Hematology,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan Shandong 250013,China)

机构地区：[1]山东中医药大学第一临床医学院,山东济南250013 [2]山东中医药大学附属医院血液病科,山东济南250013

出　　处：《新中医》2025年第3期9-16,共8页New Chinese Medicine

摘　　要：目的:通过收集包含虫类药处方治疗骨髓增殖性疾病(MPD)的文献,探讨虫类药治疗MPD的应用规律和作用机制。方法:在中国知网(CNKI)收集并筛选自建库起至2023年12月期间在学术期刊发表的含虫类药处方治疗MPD的文献,统计分析处方中虫类药的使用频次、四气五味、归经和毒性,并进行关联规则分析,总结出虫类药治疗MPD的应用规律和核心药对。通过中药分子机制生物信息学分析工具(BATMANTCM)检索,并结合现有文献筛选核心药对的活性成分和作用靶点。使用人类基因数据库(GeneCards)、在线人类孟德尔遗传数据库(OMIM)以及疾病相关的基因与突变位点数据库(DisGeNET)检索MPD的疾病靶点。将核心药对的作用靶点与疾病靶点取交集,并将核心药对的活性成分和交集靶点导入Cytoscape3.9.1软件中进行蛋白质相互作用(PPI)网络构建分析,筛选核心成分。借助STRING数据库进行PPI网络构建,筛选核心靶点。通过DAVID数据库对交集靶点进行基因本体(GO)功能富集分析与京都基因与基因组百科全书(KEGG)通路富集分析。将筛选出的核心成分与核心靶点进行分子对接。结果:收集到关于虫类药治疗MPD的文献共166篇,其中水蛭的使用频次最高,共计103次。所用的虫类药,药性、药味主要为寒性、平性,咸味,归经主要归肝经,毒性为无毒或有小毒。支持度最高的药物组合为水蛭-地龙。水蛭-地龙药对中有50个活性成分,共对应585个作用靶点,与MPD的交集靶点有156个。筛选出的核心成分为熊果酸、花生四烯酸,核心靶点为细胞肿瘤抗原p53 (TP53)、RAC-α丝氨酸/苏氨酸蛋白激酶(AKT1)。GO功能富集分析结果主要涉及调控细胞内关键过程、应对外来刺激、涉及分子间相互作用、维持细胞正常生理及对外界的适应性等,KEGG通路富集分析结果主要涉及癌症、动脉粥样硬化及细胞凋亡等多条疾病相关通路。将筛选出Objective:To investigate the application and mechanism of Insect medicinals in the treatment of myeloproliferative diseases(MPD)by collecting literatures containing Insect medicinals prescription.Methods:The literatures on the treatment of MPD with Insect medicinals published in academic journals from the self-established database to December 2023 were collected and screened on CNKI.The frequency of use,four properties,five flavours,meridian tropism and toxicity of Insect medicinals in the prescription were statistically analyzed,and the association rules were analyzed to summarize the application rules and core drug pairs of Insect medicinals in the treatment of MPD.The active ingredients and action targets of the core drug pairs were screened by the A Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine(BATMAN-TCM)combined with the existing literature.Disease targets of MPD were searched using the Human Gene Database(GeneCards),the online Mendelian Inheritance in Man(OMIM),and the Disease-Associated Genes and Mutation Sites Database(DisGeNET).The target of the core drug pair was intersected with the disease target,and the active components of the core drug pair and the intersection targets were imported into Cytoscape3.9.1 software for protein-protein interaction(PPI)network construction analysis,and the core components were screened.PPI network construction with STRING database,Screening core targets.The Gene Ontology(GO)function enrichment analysis and the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed for the intersection targets using the DAVID database.Molecular docking was performed between the screened core components and the core target.Results:A total of 166 literatures on the treatment of MPD were collected,among which Hirudo were used the most frequently,103 times in total.The Insect medicinals used are mainly cold,neutral,salty,mainly liver channel,toxic is non-toxic or small.The drug combination with the highest support was Hi

关 键 词：骨髓增殖性疾病 虫类药 数据挖掘 网络药理学 分子对接 作用机制 

分 类 号：R273[医药卫生—中西医结合] R733.3[医药卫生—中医肿瘤科]