口乳杆菌BE7N改善db/db小鼠脂代谢异常的研究  

Effects of Lactobacillus oris BE7N on lipid metabolism in db/db mice

作　　者：陈怡 栾哲 赵义名 王淑芳 黄园园[3] 孙刚 CHEN Yi;LUAN Zhe;ZHAO Yiming;WANG Shufang;HUANG Yuanyuan;SUN Gang(Department of Gastroenterology and Hepatology,the First Medical Center,Chinese PLA General Hospital,Beijing 100853;Department of Gastroenterology and Hepatology,Hainan Hospital,Chinese PLA General Hospital;Key Laboratory of Animal Ecology and Conservation Biology,Institute of Zoology,Chinese Academy of Sciences,China)

机构地区：[1]中国人民解放军总医院第一医学中心消化内科医学部,北京100853 [2]中国人民解放军总医院海南医院消化内科 [3]中国科学院动物研究所

出　　处：《胃肠病学和肝病学杂志》2025年第2期208-212,共5页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家自然科学基金项目(32170898)。

摘　　要：目的研究口乳杆菌BE7N(Lactobacillus oris BE7N,L.oris BE7N)对db/db小鼠脂代谢的影响。方法将db/db小鼠随机分为空白对照组和L.oris BE7N干预组,灌胃处理4周,期间监测体质量、摄食量和肛温情况。称重肝脏和脂肪组织;HE染色观察肝脏和棕色脂肪组织形态改变;检测血清中TC、TG、LDL-C、HDL-C水平;RT-PCR检测棕色脂肪组织中PPARγ、PGC-1α、UCP1、PRDM16、ELOVL3 mRNA的表达。结果与空白对照组相比,L.oris BE7N干预可显著延缓db/db小鼠体质量增加(P<0.01),提高小鼠核心体温(P<0.05),降低肝脏和内脏脂肪组织湿重(P<0.05),降低外周血中TC和LDL-C含量(P<0.05);减轻肝脏和棕色脂肪组织的脂质沉积;L.oris BE7N干预显著提高db/db小鼠棕色脂肪组织产热相关基因PPARγ、PGC-1α、UCP1、PRDM16和ELOVL3的mRNA表达水平(P<0.05)。结论L.oris BE7N可以通过调节棕色脂肪组织PPARγ/PGC-1α/UCP1信号通路,减轻脂肪沉积,改善db/db小鼠脂代谢异常。Objective To investigate the effects of Lactobacillus oris BE7N(L.oris BE7N)on lipid metabolism in db/db mice.Methods Db/db mice were randomly divided into control group and L.oris BE7N intervention group.Both groups received oral gavage for 4 weeks.Body weight,food intake,and rectal temperature were monitored during the study.Liverand adipose tissues were weighed.Morphology changes in the liver and brown adipose tissue were assessed using HE staining.Serum levels of TC,TG,LDL-C,and HDL-C were measured and the levels of PPARγ,PGC-1α,UCP1,PRDM16 and ELOVL3 mRNA in brown adipose tissue were detected by RT-PCR.Results Compared with the control group,L.oris BE7N intervention significantly slowed body weight gain in db/db mice(P<0.01),increased core body temperature(P<0.05),reduced liver and visceral fat mass(P<0.05),and decreased serum TC and LDL-C levels(P<0.05).Lipid deposition in the liver and brown adipose tissue was alleviated,and L.oris BE7N significantly increased the levels of thermogenesis-related genes,including PPARγ,PGC-1α,UCP1,PRDM16 and ELOVL3 in brown adipose tissue(P<0.05).Conclusion L.oris BE7N can improve lipid metabolism disorders and reduce fat deposition in db/db mice by regulating the PPARγ/PGC-1α/UCP1 signaling pathway in brown adipose tissue.

关 键 词：乳杆菌 肥胖 棕色脂肪组织 产热 

分 类 号：R589.2[医药卫生—内分泌] R57[医药卫生—内科学]