An Array-based Photolithographically Patterned Electrochemical Sensing Platform for Highly Sensitive Determination of Uric Acid,Dopamine,L-Tryptophan,and Pyridoxine in Biological Samples  

作　　者：Ankit Kumar Singh Shreanshi Agrahari Shivani Shukla Ida Tiwari Muhammad Ahmad S.Ravi P.Silva 

机构地区：[1]Department of Chemistry(Centre of Advanced Study),Institute of Science,Banaras Hindu University,Varanasi,221005,India [2]Advanced Technology Institute,University of Surrey,Guildford,Surrey,GU27XH,UK

出　　处：《Journal of Analysis and Testing》2024年第4期505-517,共13页分析检测(英文)

基　　金：The author AS(Chem./2018-19/RET/Sept.18term/1/4809),SA(Chem./2019-2020/RET-2/Sept.19-term/1/975)and SS(Chem.-22/RET-Ex./July-22-term/26/564)are grateful to UGC,New Delhi and IOE,BHU for funding their doctoral research;The financial supports received from Scheme for Promotion of Academic and Research Collaboration(SPARC-6019);MHRD,India,UKIERI,UK and IOE incentive grant for faculty(Scheme Number-6031)。

摘　　要：Biomolecules play important roles in physiological functions and pharmacological characteristics of human body.Uric acid(UA)is the end product of purine.Dopamine(DA)is a neurotransmitter of catecholamine group.L-tryptophan is an essential amino acid that can be metabolized to neuroactive substances.Pyridoxine is a water-soluble vitamin playing an important role in nervous system.The abnormalities in their concentration levels led to a wide range of signifi cant mental and physical illnesses.Thus,electrochemical analysis of these analytes on an array system would be benefi cial from clinical or scientifi c points of view.This work was aimed at the development of practical sensor array for determination of multiple analytes on a single sensing platform using individually addressable microelectrodes.The occurrence of adsorption–desorption phenomenon on the surface of palladium microelectrode array(Pd MEA)printed on the silicon wafer through photolithography was exploited for electro-oxidation of UA,DA,L-tryptophan and pyridoxine.The sensing of electroactive UA was done using carbon nanotubes(CNTs)grown Pd MEA as a working electrode,while selectivity for other analytes was achieved by the modifi cation of CNTs/Pd MEA through electrodeposition of poly(L-lysine)(poly(L-lysine)/CNTs/Pd MEA)for DA sensing,poly(L-arginine)(poly(L-arginine)/CNTs/Pd MEA)for L-tryptophan sensing and reduced graphene oxide(rGO/CNTs/Pd MEA)for pyridoxine sensing.The electrochemical diff erential pulse voltammetry(DPV)analyses reveal excellent linearity in the concentration ranges of 50–6000μmol/L,2–8000μmol/L,20–15,000μmol/L,and 10–5000μmol/L with detection limits of 15.0,0.5,10.0,and 1.0μmol/L for UA,DA,L-tryptophan,and pyridoxine,respectively.The proposed multiple analytes sensor has shown very high sensitivities of 140,9580,2280,and 940μA·(μmol·L^(-1))^(-1)·cm^(-2)for UA,DA,L-tryptophan,and pyridoxine sensing,respectively.Further,accuracy and reliability of the fabricated sensor were also tested in real samples.

关 键 词：Microelectrode arrays Multianalyte sensor Carbon nanotubes Uric acid DOPAMINE L-TRYPTOPHAN PYRIDOXINE 

分 类 号：Q503[生物学—生物化学] O657.1[理学—分析化学]