复方醋酸氯己定喷雾剂的制备及体外抑菌活性研究  

Study of in vitro Antimicrobial Efficacy and Preparation of Compound Chlorhexidine Acetate Spray

作　　者：王文习 冯荣伟 李云霞 许剑峰 刘军 杨静 WANG Wenxi;FENG Rongwei;LI Yunxia;XU Jianfeng;LIU Jun;YANG Jing(Departmant of Pharmacy,NO.980 Hospital of Joint Logistical Support Force,Shijiazhuang Hebei 050082,China)

机构地区：[1]联勤保障部队980医院药剂科,河北石家庄050082 [2]联勤保障部队980医院档案科,河北石家庄050082 [3]郑州联勤保障中心药品仪器监督检验站

出　　处：《联勤军事医学》2024年第11期922-927,共6页Military Medicine of Joint Logistics

基　　金：2023年度河北省医学科学研究课题计划(20231306)。

摘　　要：目的 考察复方醋酸氯己定喷雾剂的制备及体外抑菌活性。方法 制备复方醋酸氯己定喷雾剂;采用滤纸片抑菌圈法,将复方醋酸氯己定喷雾剂中各组分对铜绿假单胞菌、金黄色葡萄球菌、生孢梭菌和白色念珠菌4株标准菌株进行最小抑制浓度(minimum inhibitory concentration,MIC)测定;使用相同的方法,以醋酸氯己定溶液(联勤保障部队980医院自制制剂)和甲硝唑对照溶液(0.05 mg/ml)作为对照,检测复方醋酸氯己定喷雾剂对4种标准菌株的抑菌活性。结果 本研究制备了3个批次的复方醋酸氯己定喷雾剂,最终获得复方醋酸氯己定喷雾剂中醋酸氯己定含量分别为标示量的99.81%、101.67%、103.09%,聚乙烯醇含量分别为标示含量的102.62%、100.18%、104.43%,甲硝唑含量分别为标示含量的100.89%、103.33%、102.82%,3个批次的复方醋酸氯己定喷雾剂的质量检验结果均符合规定。复方醋酸氯己定喷雾剂、醋酸氯己定溶液对白色念珠菌、金黄色葡萄球、铜绿假单胞菌的抑菌圈直径较甲硝唑对照溶液明显增大(P均<0.05);复方醋酸氯己定喷雾剂、甲硝唑对照溶液对生孢梭菌的抑菌圈直径较醋酸氯己定溶液明显增大(P均<0.05)。结论 复方醋酸氯己定喷雾剂配方中的有效抑菌组分为醋酸氯己定和甲硝唑,二者各自的抑菌活性在和其它组分混合制剂后没有产生拮抗作用。相较一般单一组分的消毒抑菌制剂,复方醋酸氯已定喷雾剂具有广谱抑菌优势。Objective To investigate the preparation of compound chlorhexidine acetate spray and its in vitro antimicrobial activity.Methods The compound chlorhexidine acetate spray was prepared.The paper-disc agar-diffusion method was used to determine the minimum inhibitory concentration(MIC) of each component of compound chlorhexidine acetate spray against 4 standard strains:Pseudomonas aeruginosa,Staphylococcus aureus,Clostridium sporogenes and Candida albicans;the same method was used to detect the bacteriostatic activity of compound chlorhexidine acetate spray against 4 standard strains with chlorhexidine acetate solution(self-made formulation from the NO.980 hospital) and metronidazole solution(0.05 mg/ml) as the control.Results A total of 3 batches of the compound chlorhexidine acetate spray were prepared in this study,the chlorhexidine acetate content of labeled amount acetate in the compound chlorhexidine acetate spray was 99.81%,101.67% and 103.09%,respectively;the content of polyvinyl alcohol was 102.62%,100.18%,and 104.43% of the labeled content,respectively;the content of metronidazole was 100.89%,103.33%,and 102.82% of the labeled content,respectively,the quality test results of 3 batches of compound chlorhexidine acetate spray all complied with the requirements.The diameter of inhibition zones of compound chlorhexidine acetate spray and chlorhexidine acetate solution against Candida albicans,Staphylococcus aureus and Pseudomonas aeruginosa was significantly increased than that of metronidazole solution(all P<0.05);the diameter of inhibition zones of compound chlorhexidine acetate spray and metronidazole solution against Clostridium sporogenes was significantly increased than that of chlorhexidine acetate solution(all P<0.05).Conclusion The effective antimicrobial components in the formulation of compound chlorhexidine acetate spray were chlorhexidine acetate and metronidazole,with no antagonistic effect observed when mixed with other components in the formulation.Compared with typical single-component disinfe

关 键 词：醋酸氯己定 喷雾剂 甲硝唑 聚乙烯醇 涂膜剂 抑菌活性 

分 类 号：R521.7[医药卫生—内科学]