Photothermal MXene-embedded tannin-Eu^(3+)particles as in situ bacterial vaccines for accelerating healing of wounds infected by seawater immersion and providing persistent anti-infection effects  

光热响应性原位细菌疫苗敷料加速海水浸泡感染创面修复并构建持久抗感染策略

作　　者：Zhentao Li Ting Song Yanpeng Jiao Zijing Zhu Yang Liao Zonghua Liu 李振涛;宋婷;焦延鹏;朱姿婧;廖扬;刘宗华(暨南大学材料科学与工程系,广州市510632;暨南大学生物医学工程系,广州市510632;中国人民解放军南部战区总医院检验科,广州市510010)

机构地区：[1]Department of Materials Science and Engineering,Jinan University,Guangzhou 510632,China RESEARCH ARTICLE [2]Department of Biomedical Engineering,Jinan University,Guangzhou 510632,China [3]Department of Laboratory Medicine,General Hospital of Southern Theatre Command of PLA,Guangzhou 510010,China

出　　处：《Bio-Design and Manufacturing》2025年第1期116-133,I0045-I0050,共24页生物设计与制造(英文)

基　　金：supported by the National Key Research and Development Program of China(No.2018YFC0311103);the Project of Science and Technology Innovation Cultivation for University Students of Guangdong Province(No.pdjh202010062);the Science and Technology Project of Guangzhou City(No.2018020100);the General Program of China Postdoctoral Science Foundation(No.2021M701599);the National Natural Science Foundation of China(No.32201083);the Science and Technology Program of Heyuan,China(No.230510171473326);the Science and Technology Program of Guangzhou,China(No.2024A03J0232);the Medical Scientific Research Foundation of Guangdong Province of China(No.A2020548);the Fundamental Research Funds for the Central Universities,China(No.21623403).

摘　　要：The low temperature,high salt content,and bacterial composition of a seawater environment can induce severe infections in open wounds,thus impeding wound healing.To date,numerous wound dressings have been developed for injuries and various antibacterial functions.However,ordinary antibacterial strategies could not provide long-term resistance to infections and could not promote wound healing.Here,we proposed a strategy for enhancing resistance to seawater immersion wound infection by killing bacteria and delivering bacterial antigens in situ.Specifically,MXene-embedded tannin-Eu^(3+)(M@TA-Eu)particles were constructed to effectively alleviate injuries infected through seawater immersion and persistently fight infection by forming in situ bacterial vaccines and immune memory.In the particle,the platform composed of TA and Eu3+exhibited provascularization and antigen presentation effects.MXenes with near-infrared photothermal effect were introduced to kill the bacteria,promote the recruitment of antigen presentation cells,and ultimately enhance vaccination efficacy.Experimental results showed that the particles not only effectively accelerated the healing of injuries by relieving wound inflammation and inhibiting bacteria but also produced a potent vaccination by forming in situ bacterial vaccines.Therefore,the M@TA-Eu particles are novel materials for high-grade anti-infection dressing.海水的低温、高盐及复杂的细菌组成容易引起开放性创面的严重感染,进而干扰创面的愈合进程。目前虽构建了多种具备修复及抗感染功能的创面敷料,但常规的抗菌策略不能为创面提供持久的抗感染能力。针对海水浸泡创面,本研究基于杀灭细菌并原位递送细菌抗原的理念,为感染机体构筑了持久的抗感染能力。具体来说,本研究制备了一种嵌入MXene的单宁-Eu^(3+)颗粒(M@TA-Eu),其不仅能够有效促进海水浸泡感染创面的修复,还能够通过形成原位细菌疫苗和免疫记忆达到持续抗感染的效果。其中,单宁和Eu^(3+)构建的功能平台具备促血管化和抗原呈递作用,而具备光热杀菌性能的MXene能够促进抗原呈递细胞的招募,从而增强疫苗接种效果。研究结果显示,该功能颗粒不仅能够通过缓解创面炎症和抑制创面感染加速创面愈合,还能够通过形成原位细菌疫苗,产生持久的免疫效果。因此,M@TA-Eu颗粒有望成为一种具备持久抗感染能力的高效敷料选择。

关 键 词：MXene TANNIN Wound healing ANTI-INFECTION In situ vaccine 

分 类 号：TG1[金属学及工艺—金属学]