Phenanthrene perturbs hematopoietic development and causes hematopoietic defects in zebrafish  

作　　者：Lingyu Ren Yue Wang Ying Ren Guangke Li Nan Sang 

机构地区：[1]College of Environment and Resource,Research Center of Environment and Health,Shanxi University,Taiyuan 030006,China

出　　处：《Journal of Environmental Sciences》2025年第5期573-581,共9页环境科学学报(英文版)

基　　金：supported by the National Natural Science Foundation of China(Nos.22276117 and 22076108);the Science and Technology Innovation Talent Team Project of Shanxi Province(No.202204051002024).

摘　　要：Phenanthrene(Phe)is one of the common polycyclic aromatic hydrocarbons in the environment,and recent studies show that it can cause cardiac developmental toxicity and immunotoxicity.However,it is still unknown whether it can affect the hematopoietic development in aquatic organisms.To address this question,zebrafish(Danio rerio)were chronically exposed to Phe at different concentrations.We found that Phe caused structural damage to the renal tubules in the kidney,induced malformed erythrocytes in peripheral blood,and decreased the proportion of myeloid cells in adult zebrafish,suggesting possible negative impacts that Phe posed to hematopoietic development.Then,using in situ hybridization technology,we found that Phe decreased the expression of primitive hematopoietic marker genes,specifically gata1 and pu.1,accompanied by an obstruction of primitive erythrocyte circulation.Furthermore,Phe impaired definitive hematopoiesis,increased aberrations of the transient hematopoietic site(PBI),and reduced the generation of hematopoietic stem cells,ultimately influencing the number of erythrocytes and myeloid cells.The findings suggested that Phe could induce hematopoietic toxicity in zebrafish embryos and pose unknown ecological risks.

关 键 词：PHENANTHRENE ZEBRAFISH Hematopoietic development Hematopoietic defect 

分 类 号：R994.6[医药卫生—毒理学]