Transcriptomic Analysis and Computational Study Provide Insights into the Mechanism of Anti-tumor Activity of CuB and CuE on HepG2 cells  

作　　者：Ru CHEN Wuyi BAN Shitong SONG Haoran MA Yuxin ZHENG Lei SONG 

机构地区：[1]College of Pharmacy,Southwest Minzu University,Chengdu 610225,China

出　　处：《Medicinal Plant》2025年第1期39-48,共10页药用植物(英文)

摘　　要：[Objectives]To study the effect of carcinoma cell line(HepG2)and mechanism of anti-tumor activity of cucurbitacin B(CuB)and cucurbitacin E(CuE)on HepG2 cells.[Methods]HepG2 cells were treated with various concentrations of CuB and CuE,and the proliferation,cell cycle distribution,apoptosis,and performed RNA-seqtranscriptomics of these cells were evaluated after treatment.[Results]The results demonstrated that,in comparison to the control group,HepG2 cells treated with CuB and CuE displayed a synergistic effect on growth inhibition,cell cycle arrest at the G 2/M phase,and apoptosis induction in a concentration-and time-dependent manner.Western blotting analysis using protein extracts derived from HepG2 cells showed that CuB and CuE induced apoptosis by increasing the expression of endogenous levels of full-length caspase-3(35 kDa)and the large fragment of cleaved caspase-3(17 kDa).Moreover,transcriptomic results demonstrated that after treatment,the differentially expressed genes associated with pathways such as"cell cycle","spliceosome","metabolic pathways",and"carbon metabolism"were significantly up-/down-regulated.[Conclusions]These results demonstrate that the anti-tumor mechanisms of CuB and CuE are attributed to their interference in cell cycle and metabolism processes.Collectively,the treatment with CuB and CuE may serve as a promising therapeutic option for hepatocellular carcinoma.

关 键 词：TRANSCRIPTOMIC Cucurbitacin B Cucurbitacin E HepG2 cells 

分 类 号：R73[医药卫生—肿瘤]