Impact of residual antibiotics on microbial decomposition of livestock manures in Eutric Regosol:Implications for sustainable nutrient recycling and soil carbon sequestration  

作　　者：Linfa Fang Prakash Lakshmanan Xiaoxuan Su Yujia Shi Zheng Chen Yu Zhang Wei Sun Junxi Wu Ran Xiao Xinping Chen 

机构地区：[1]Interdisciplinary Research Center for Agriculture Green Development in Yangtze River Basin,College of Resources and Environment,Southwest University,Chongqing 400715,China [2]Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi),Ministry of Agriculture and Rural Affairs,Guangxi Key Laboratory of Sugarcane Genetic Improvement,Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China [3]Queensland Alliance for Agriculture and Food Innovation,University of Queensland,St Lucia 4067,QLD,Australia [4]Key Laboratory of Ecosystem Network Observation and Modeling,Institute of Geographic Sciences and National Resources Research,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Journal of Environmental Sciences》2025年第1期498-511,共14页环境科学学报(英文版)

基　　金：supported by the National Natural Science Foundation of China(No.U20A2047);the Key Research and Development Project for Tibet Autonomous Region(No.XZ202201ZY0003N);the National Key Research and Development Program of China(No.2022YFD1901402);the Lasa Science and Technology Bureau(No.LSKJ202206);the Foundation of Graduate Research and Innovation in Chongqing(No.CYB22127).

摘　　要：The land application of livestock manure has been widely acknowledged as a beneficial approach for nutrient recycling and environmental protection.However,the impact of residual antibiotics,a common contaminant of manure,on the degradation of organic compounds and nutrient release in Eutric Regosol is not well understood.Here,we studied,how oxytetracycline(OTC)and ciprofloxacin(CIP)affect the decomposition,microbial community structure,extracellular enzyme activities and nutrient release from cattle and pig manure using litterbag incubation experiments.Results showed that OTC and CIP greatly inhibited livestock manure decomposition,causing a decreased rate of carbon(28%-87%),nitrogen(15%-44%)and phosphorus(26%-43%)release.The relative abundance of gramnegative(G-)bacteria was reduced by 4.0%-13%while fungi increased by 7.0%-71%during a 28-day incubation period.Co-occurrence network analysis showed that antibiotic exposure disrupted microbial interactions,particularly among G-bacteria,G+bacteria,and actinomycetes.These changes in microbial community structure and function resulted in decreased activity of urease,β-1,4-N-acetyl-glucosaminidase,alkaline protease,chitinase,and catalase,causing reduced decomposition and nutrient release in cattle and pig ma-nures.These findings advance our understanding of decomposition and nutrient recycling from manure-contaminated antibiotics,which will help facilitate sustainable agricultural production and soil carbon sequestration.

关 键 词：Residual antibiotics Livestock manure decomposition Microbial community Co-occurrence network Enzyme activities 

分 类 号：S15[农业科学—土壤学]