Disentangling microbial coupled fillers mechanisms for the permeable layer optimization process in multi-soil-layering systems  

作　　者：Daxin Sun Chuanping Feng Yongheng Zhan Bingbing Deng Duoduo Mei Nan Chen Weiwu Hu 

机构地区：[1]School of Water Resources and Environment,MOE Key Laboratory of Groundwater Circulation and Environmental Evolution,China University of Geosciences(Beijing),Beijing 100083,China [2]Journal Center,China University of Geosciences(Beijing),Beijing 100083,China

出　　处：《Journal of Environmental Sciences》2025年第1期538-549,共12页环境科学学报(英文版)

基　　金：supported by the National Natural Science Foundation of China(No.42077163).

摘　　要：Themulti-soil-layering(MSL)systems is an emerging solution for environmentally-friendly and cost-effective treatment of decentralized rural domestic wastewater.However,the role of the seemingly simple permeable layer has been overlooked,potentially holding the breakthroughs or directions to addressing suboptimal nitrogen removal performance in MSL systems.In this paper,the mechanism among diverse substrates(zeolite,green zeolite and biological ceramsite)coupled microorganisms in different systems(activated bacterial powder and activated sludge)for rural domestic wastewater purification was investigated.The removal efficiencies performed by zeolite coupled with microorganisms within 3 days were 93.8%for COD,97.1%for TP,and 98.8%for NH_(4)^(+)-N.Notably,activated sludge showed better nitrification and comprehensive performance than specialized nitrifying bacteria powder.Zeolite attained an impressive 89.4%NH4+-N desorption efficiency,with a substantive fraction of NH_(4)^(+)-N manifesting as exchanged ammonium.High-throughput 16S rRNA gene sequencing revealed that aerobic and parthenogenetic anaerobic bacteria dominated the reactor,with anaerobic bacteria conspicuously absent.And the heterotrophic nitrificationaerobic denitrification(HN-AD)process was significant,with the presence of denitrifying phosphorus-accumulating organisms(DPAOs)for simultaneous nitrogen and phosphorus removal.This study not only raises awareness about the importance of the permeable layer and enhances comprehension of the HN-AD mechanism in MSL systems,but also provides valuable insights for optimizing MSL system construction,operation,and rural domestic wastewater treatment.

关 键 词：Rural domestic wastewater Multi-soil-layering(MSL)system Heterotrophic nitrification-aerobic denitrification(HN-AD) Nitrogen and phosphorus removal Microbial evolution 

分 类 号：X70[环境科学与工程—环境工程]