口腔血链球菌提取物对SKOV3细胞的抑制作用  

Inhibitory Effect of Oral Streptococcus Sanguis Extract on SKOV3 Cells

作　　者：孙膀 马晟利[2] 葛文玉 刘勇韬 SUN Pang;MA Shengli;GE Wenyu;LIU Yongtao(Jiamusi University,Jiamusi 154002,China;Stomatology Center of Heilongjiang Provincial Hospital,Harbin 150036,China)

机构地区：[1]佳木斯大学,黑龙江佳木斯154002 [2]黑龙江省医院口腔医学中心,黑龙江哈尔滨150036

出　　处：《口腔医学研究》2025年第2期140-146,共7页Journal of Oral Science Research

摘　　要：目的:血链球菌提取物XLS是从口腔血链球菌中提取的具有一定生物活性作用的细菌素样物质。本研究旨在探讨XLS对卵巢癌(ovarian cancer,OC)SKOV3细胞系的抑制作用及其可能的分子机制,为后续对XLS的相关研究和应用提供理论依据。方法:(1)采用CCK-8法检测XLS和紫杉醇分别对SKOV3细胞活性的影响;(2)通过克隆形成、Transwell、细胞划痕实验检测XLS对细胞增殖、侵袭和迁移的影响;(3)使用Western blot和实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qPCR)分析XLS对磷脂酰肌醇3激酶/蛋白激酶B(phosphatidylinositol 3 kinase/protein kinase B,PI3K/Akt)通路及相关蛋白表达的调控作用;(4)检测线粒体膜电位、活性氧(reactive oxygen species,ROS)和Ca^(2+)水平以探讨XLS诱导的线粒体凋亡。结果:(1)经XLS处理后SKOV3细胞的活力随XLS浓度的增加而下降;(2)XLS表现出浓度依赖性抑制SKOV3细胞增殖,侵袭和迁移;(3)本实验中XLS能抑制PI3K/Akt信号通路的激活,调节相关基因蛋白的表达,诱导细胞凋亡;(4)XLS可降低线粒体膜电位,增加氧化应激和Ca^(2+)失衡,进一步破坏细胞结构和功能。结论:XLS能够抑制SKOV3细胞的增殖、侵袭和迁移,诱导凋亡,表现出显著抑制SKOV3细胞的潜力。Objective:To investigate the inhibitory effect of Xue Lian Su(XLS),a kind of bacteriocin-like substance extracted from oral streptococcus sanguis,on ovarian cancer(OC)SKOV3 cell line and its possible molecular mechanism.Methods:The effects of XLS and paclitaxel on the activity of SKOV3 cells were detected by CCK-8 method.The effects of XLS on cell proliferation,invasion,and migration were detected by colony formation,Transwell,and cell scratch.Western blot and quantitative real-time polymerase chain reaction(qPCR)were used to analyze the regulation of XLS on phosphatidylinositol 3 kinase/protein kinase B(PI3K/Akt)pathway and related protein expression.Mitochondrial membrane potential,reactive oxygen species(ROS),and Ca 2+levels were detected to investigate XLS-induced mitochondrial apoptosis.Results:The viability of SKOV3 cells treated with XLS decreased with the increase of XLS concentration.XLS inhibited the proliferation,invasion,and migration of SKOV3 cells in a concentration-dependent manner.XLS inhibited the activation of PI3K/Akt signaling pathway,regulated the expression of related gene proteins,and induced apoptosis.XLS could reduce mitochondrial membrane potential,increase oxidative stress and Ca 2+imbalance,and further destroy cell structure and function.Conclusion:XLS can inhibit the proliferation,invasion,and migration of SKOV3 cells and induce apoptosis,showing the potential to significantly inhibit SKOV3 cells.

关 键 词：XLS SKOV3细胞 增殖 迁移 侵袭 

分 类 号：R78[医药卫生—口腔医学] R737.31[医药卫生—临床医学]