基于液相色谱-串联质谱的坐骨神经痛模型大鼠脊髓蛋白质组学分析  

Liquid chromatography-tandem mass spectrometry-based proteomics analysis of the spinal cord of rats with sciatica model

作　　者：唐甸峰 范佳佳 白凤媛 蔡仁军 刘涛 徐秋玲 TANG Dian-feng;FAN Jia-jia;BAI Feng-yuan;CAI Ren-jun;LIU Tao;XU Qiu-ling(Department of Traditional Chinese Medicine,The First Affiliated Hospital of Hainan Medical University,Haikou 570102,China;Hainan Medical University of Traditional Chinese Medicine,Haikou 571199,China;Department of Acupuncture and Moxibustion,Geriatric Hospital of Hainan,Haikou 570311,China)

机构地区：[1]海南医科大学第一附属医院中医科,海口570102 [2]海南医科大学中医学院,海口571199 [3]海南省老年病医院针灸科,海口570311

出　　处：《中国疼痛医学杂志》2025年第2期93-101,共9页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金委员会地区科学基金项目(81660820);海南省自然科学基金高层次人才项目(821RC571);海南医学院研究生创新课题项目(HYYB2023A10)。

摘　　要：目的:采用蛋白质组学技术观察正常组和坐骨神经痛模型大鼠脊髓蛋白标志物,以及各差异蛋白所参与的生物学过程,探讨坐骨神经痛的发生机制。方法:将12只SD雄性大鼠按简单随机抽样法分为正常组和模型组,每组6只。正常组不做任何处理,模型组在大鼠左侧坐骨神经进行结扎造模,每组3只用于定量蛋白质组学鉴定蛋白标志物并进行生物信息学分析。结果:从大鼠脊髓组织中共鉴定到5975个蛋白,其中正常组与模型组间有51个差异蛋白(differentially expressed proteins,DEPs),与正常组相比,模型组中有9个蛋白表达增加,42个蛋白表达降低。其中Cyp51a1和Cad表达下降。KEGG富集分析结果显示,差异蛋白主要涉及丙酮酸代谢、辅助因子生物合成中的谷氨酸和谷氨酰胺代谢、胰岛素等信号通路。结论:坐骨神经痛的产生机制可能与Cyp51a1、Cad的表达,以及谷氨酸和谷氨酰胺的代谢存在一定的关系。Objective:To observe the spinal cord protein markers in normal and sciatica model rats,as well as the biological processes involved in each differential protein with proteomics technology,and explore the mech-anism of sciatica.Methods:Twelve SD male rats were simply randomized into normal group and model group,six in each group.The rats in model group were ligated and modeled in the left sciatic nerve,and 3 rats in each group were used for quantitative proteomics to identify protein markers and for bioinformatics analysis.Results:A total of 5975 proteins were identified from rats spinal cord tissues,among which 51 differentially expressed proteins(DEPs)were found between the normal group and the model group,and 9 proteins with increased expression and 42 proteins with decreased expression were found in the model group compared with the normal group.Among them,Cyp51a1 and Cad expression were decreased.The KEGG enrichment analysis showed that the DEPs were mainly involved in signaling pathways such as pyruvate metabolism,glutamate and glutamine metabolism in cofactor biosynthesis,and insulin.Conclusion:The mechanism of sciatica may be related to the expression of Cyp51a1,Cad and the metabolism of glutamate and glutamine.

关 键 词：坐骨神经痛 蛋白质组学 液相色谱-串联质谱 

分 类 号：R-332[医药卫生] R745.11