Effects of reductive soil disinfestation on potential pathogens and antibiotic resistance genes in soil  

作　　者：Huijuan Duan Yue Yin Yifei Wang Zhelun Liu Tiangui Cai Dong Zhu Chun Chen Guilan Duan 

机构地区：[1]State Key Lab of Urban and Regional Ecology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China [2]Institute of Urban Environment,Chinese Academy of Sciences,Xiamen 361021,China [3]College of Resources and Environment,Huazhong Agricultural University,Wuhan 430070,China [4]State Key Laboratory of Crop Stress Biology for Arid Areas,Shaanxi Key Laboratory of Agricultural and Environmental Microbiology,College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China [5]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Environmental Sciences》2025年第4期373-384,共12页环境科学学报(英文版)

基　　金：supported by the Key Research and Development Program of Shandong Province,China(No 2021CXGC010803);Pan’an County Chinese Medicine Industry Project(No.PZYF202103).

摘　　要：Reductive soil disinfestation(RSD)is commonly employed for soil remediation in greenhouse cultivation.However,its influence on antibiotic resistance genes(ARGs)in soil remains uncertain.This study investigated the dynamic changes in soil communities,potential bacterial pathogens,and ARG profiles under various organicmaterial treatments during RSD,including distillers’grains,potato peel,peanut vine,and peanut vine combined with charcoal.Results revealed that applying diverse organic materials in RSD significantly altered bacterial community composition and diminished the relative abundance of potential bacterial pathogens(P<0.05).The relative abundance of high-risk ARGs decreased by 10.7%-30.6%after RSD treatments,the main decreased ARG subtypeswere AAC(3)_Via,dfrA1,ErmB,lnuB,aadA.Actinobacteria was the primary host of ARGs and was suppressed by RSD.Soil physicochemical properties,such as total nitrogen,soil pH,total carbon,were crucial factors affecting ARG profiles.Our findings demonstrated that RSD treatment inhibited pathogenic bacteria and could be an option for reducing high-risk ARG proliferation in soil.

关 键 词：Reductive soil disinfestation(RSD) Antibiotic resistance genes(ARGs) Bacterial communities Farmland soil Potential pathogens 

分 类 号：R73[医药卫生—肿瘤]