血清CTRP3、脂联素的表达水平与溃疡性结肠炎严重程度的相关性  

Correlation between the level of serum CTRP3,adiponectin expression and the severity of ulcerative colitis

作　　者：吕志文 桂春喜 何彦春 游济洲 LYU Zhi-wen;GUI Chun-xi;HE Yan-chun(Department of Laboratory,Honghu Traditional Chinese Medicine Hospital,Honghu Hubei 433200,China;Department of Rheumatology and Immunology,Honghu Traditional Chinese Medicine Hospital,Honghu Hubei 433200,China)

机构地区：[1]洪湖市中医医院检验科,湖北洪湖433200 [2]洪湖市中医医院风湿免疫科,湖北洪湖433200

出　　处：《临床和实验医学杂志》2025年第1期66-69,共4页Journal of Clinical and Experimental Medicine

基　　金：湖北省卫健委科研课题(编号:2023010)。

摘　　要：目的 观察血清补体C1q肿瘤坏死因子相关蛋白3(CTRP3)、脂联素的表达水平与溃疡性结肠炎(UC)严重程度的相关性。方法 纳入2022年1月至2023年12月洪湖市中医医院接收的150例UC患者进行前瞻性研究,作为UC组,按照病情严重程度将患者分为缓解期组(n=47)、轻度活动期组(n=43)、中度活动期组(n=36)和重度活动期组(n=24)。选择同期来本院体检的健康者150名为对照组。采用酶联免疫吸附试验法检测血清CTRP3、脂联素、白细胞介素(IL)-6、肿瘤坏死因子α(TNF-α)表达水平,比较UC组和对照组、不同严重程度患者的血清CTRP3、脂联素以及炎症因子水平。采用Pearson相关系数法分析UC患者血清CTRP3、脂联素表达与炎症因子的相关性;采用多因素Logistic回归分析对影响患者发生UC的因素进行分析;采用受试者操作特征(ROC)曲线分析血清CTRP3、脂联素对UC患者疾病活动度的预测价值。结果 UC组CTRP3水平为(137.53±44.48) ng/mL,低于对照组[(319.84±53.78) ng/mL],脂联素、IL-6、TNF-α水平分别为(14.32±4.62)、(47.99±13.09)、(40.61±12.65) pg/mL,均高于对照组[(7.54±2.60)、(22.02±4.98)、(20.48±3.78) pg/mL],差异均有统计学意义(P<0.05)。缓解期组、轻度活动期组、中度活动期组及重度活动期组患者血清CTRP3表达水平依次降低,脂联素表达水平依次升高,差异均有统计学意义(P<0.05)。UC患者血清CTRP3表达水平与IL-6、TNF-α均呈负相关(r=-0.607,-0.557,P<0.05),脂联素表达水平与IL-6、TNF-α均呈正相关(r=0.556,0.547,P<0.05)。血清CTRP3(OR=0.437,95%CI:0.229~0.834)低表达及脂联素(OR=1.455,95%CI:1.134~1.866)、IL-6(OR=1.268,95%CI:1.053~1.528)、TNF-α(OR=1.188,95%CI:1.030~1.371)高表达是影响UC发生的危险因素(P<0.05)。血清CTRP3联合脂联素预测UC患者疾病活动度的曲线下面积为0.930,明显高于血清CTRP3、脂联素单独检测(P<0.05),敏感度为80.58%,特异度为93.62%。结论 UC患者血清CTRP3表Objective To explor the correlation between serum complement C1q tumor necrosis factor related protein 3(CTRP3),adiponectin expression and severity of ulcerative colitis(UC).Methods A prospective study was conducted on 150 UC patients admitted to Honghu Traditional Chinese Medicine Hospital from January 2022 to December 2023,as the UC group.They were divided into the remission group(n=47),the mild active group(n=43),the moderate active group(n=36)and the severe active group(n=24)according to the severity.At the same time,150 healthy people who had routine examination in our hospital were selected as the control group.The expression levels of CTRP3,adiponectin,interleukin(IL)-6 and tumor necrosis factor-α(TNF-α)were detected by enzyme linked immunosorbent assay.The levels of serum CTRP3,adiponectin and inflammatory factors were compared between UC group and control group,and patients with different severity.The correlation between serum CTRP3,adiponectin expression and inflammatory factors IL-6 and TNF-αin patients with UC was analyzed by Pearson related coefficient.The factors affecting the occurrence of UC patients were analyzed by multivariate Logistic regression analysis.The predictive value of serum CTRP3 and adiponectin for disease activity in UC patients was analyzed by receiver operating characteristic(ROC)curve.Results The level of CTRP3 in UC group was(137.53±44.48)ng/mL,which was lower than that in control group[(319.84±53.78)ng/mL],the levels of adiponectin,IL-6 and TNF-αin UC group were(14.32±4.62),(47.99±13.09)and(40.61±12.65)pg/mL,respectively,which were higher than those in the control group[(7.54±2.60),(22.02±4.98)and(20.48±3.78)pg/mL],and the differences were statistically significant(P<0.05).In the remission group,the mild active stage group,the moderate active stage group and the severe active stage group,the expression level of serum CTRP3 decreased in turn,and the expression level of adiponectin increased in turn(P<0.05).The expression level of serum CTRP3 was negatively correlate

关 键 词：溃疡性结肠炎 脂联素 补体C1q肿瘤坏死因子相关蛋白3 严重程度 

分 类 号：R73[医药卫生—肿瘤]