基于网络药理学探讨穿心莲内酯的潜在抗炎机制  

Analysis of the potential anti-inflammatory mechanism of andrographolide based on network pharmacology

作　　者：王华 刘伟[1] Wang Hua;Liu Wei(The Third Affiliated Hospital of Xinxiang Medical University,Henan 453003,China)

机构地区：[1]新乡医学院第三附属医院药学部,453003

出　　处：《山西医药杂志》2025年第3期176-181,共6页Shanxi Medical Journal

摘　　要：目的基于网络药理学分析方法,探讨穿心莲内酯多靶点、多通路抗炎作用机制。方法通过DRAR-CPI服务器、GeneCards数据库寻找穿心莲内酯潜在的抗炎靶点,建立潜在靶点的蛋白相互关系网络,运用Cytoscape绘制关键活性成分-对应靶点网络,将筛选出核心靶点进行分子对接活性验证,并利用WebGestalt进行潜在靶点的基因本体(GO)、基因组百科全书(KEGG)富集分析。结果穿心莲内酯涉及的抗炎作用靶点有92个,其中孕酮受体基因(PGR)、RAC-α丝氨酸/苏氨酸蛋白激酶(AKT1)、糖皮质激素受体(NR3C1)、血红素氧合酶-1(HMOX1)、丝裂原活化蛋白激酶1(MAPK1)具有强烈的结合活性,通路富集分析显示穿心莲内酯抗炎作用主要涉及铂耐药、前列腺癌、细胞凋亡、孕酮介导的卵母细胞成熟和甲状腺激素信号通路等330条信号通路。结论本研究表明穿心莲内酯抗炎作用具有多靶点、多通路特点,为其进一步的临床应用和深入研究开发提供了思路和线索。Objective To explore the multiple-target and multipath anti-inflammatory mechanism of Andrographolide(AP)based on network pharmacology.Methods Retrieve the potential target proteins of AP with the aid of the DRAR-CPI server and GeneCards database,establish the corresponding target network of key active components was drawn by using Cytoscape.Verify the activity of the screened core targets through molecular docking,and the GO and KEGG enrichment analysis of potential targets was carried out by using WebGestalt.Results The analysis found that 92 targets are involved in the anti-inflammation of AP,of which PGR,AKT1,NR3C1,HMOX1 and MAPK1 showed strong binding activity,and the signalling pathways enrichment analysis showed that andrographolide anti-inflammatory effect mainly involved 330 signalling pathways,such as Platinum drug resistance,Prostate cancer,Apoptosis,Progesterone-mediated oocyte maturation,Thyroid hormone signalling pathway and so on.Conclusion This study shows that AP has a multi-target and multi-channel anti-inflammatory effect,which provides ideas and clues for its further clinical application and in-depth research and development.

关 键 词：穿心莲内酯 网络药理学 消炎药 分子对接 

分 类 号：R285[医药卫生—中药学]