An enhancer–promoter-transcription factor module orchestrates plant immune homeostasis by constraining camalexin biosynthesis  

作　　者：Ying Zhang Meng Tang Yi Zhang Qinglin Cheng Lijiang Liu Wei Chen Jiatao Xie Jiasen Cheng Yanping Fu Bo Li Daohong Jiang Xiao Yu 

机构地区：[1]National Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan,Hubei 430070,China [2]The Provincial Key Lab of Plant Pathology of Hubei Province,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China [3]Hubei Hongshan Laboratory,Wuhan,Hubei 430070,China [4]National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan),Huazhong Agricultural University,Wuhan 430070,China [5]Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture,Wuhan 430062,China

出　　处：《Molecular Plant》2025年第1期95-113,共19页分子植物(英文版)

基　　金：This research was supported by the National Natural Science Foundation of China(grant no.32200242 to X.Y.);funds from the National Key Laboratory of Agricultural Microbiology(grant no.AML2023B07 to X.Y.);the National Natural Science Foundation of China(grant no.32130087 to D.J.);the Earmarked Fund for CARS-12(to D.J.);Fundamental Research Funds for the Central Universities(grant no.2662023PY006 and2662023ZKPY001toB.L).

摘　　要：Effective plant defense against pathogens relies on highly coordinated regulation of immune gene expression.Enhancers,as cis-regulatory elements,are indispensable determinants of dynamic gene regulation,but the molecular functions in plant immunity are not well understood.In this study,we identified a novel enhancer,CORE PATTERN-INDUCED ENHANCER 35(CPIE35),which is rapidly activated upon pathogenic elicitation and negatively regulates antifungal resistance through modulating WRKY15 expression.During immune activation,CPIE35 activates the transcription of WRKY15 by forming chromatin loops with the promoter of WRKY15 in a WRKY18/40/60-,WRKY33-,and MYC2-dependent manner.WRKY15 directly binds to the promoters of PAD3 and GSTU4,suppressing their expression and leading to reduced camalexin synthesis and resistance.Interestingly,CPIE35 region is evolutionarily conserved among Brassicaceae plants,and the CPIE35-WRKY15 module exerts similar functions in Brassica napus to negatively regulate antifungal resistance.Our work reveals the“enhancer–promoter-transcription factor”regulatory mechanism in maintenance of immune homeostasis,highlighting the importance and conserved role of enhancers in fine-tuning immune gene expression in plants.

关 键 词：plant innate immunity ENHANCERS antifungal resistance transcriptional regulation cis-regulatory elements transcription factors 

分 类 号：G63[文化科学—教育学]