ALFIN-like proteins link histone H3K4me3 to H2A ubiquitination and coordinate diverse chromatin modifications in Arabidopsis  

作　　者：Xiao-Min Su Dan-Yang Yuan Na Liu Zhao-Chen Zhang Minqi Yang Lin Li She Chen Yue Zhou Xin-Jian He 

机构地区：[1]College of Life Sciences,Beijing Normal University,Beijing,100875,China [2]National Institute of Biological Sciences,Beijing 102206,China [3]State Key Laboratory of Protein and Plant Gene Research,School of Advanced Agricultural Sciences,Peking-Tsinghua Center for Life Sciences,Peking University,Beijing 100871,China [4]Tsinghua Institute of Multidisciplinary Biomedical Research,Tsinghua University,Beijing 10084,China

出　　处：《Molecular Plant》2025年第1期130-150,共21页分子植物(英文版)

基　　金：supported by the National Natural Science Foundation of China to X.-J.H.(grant numbers 32025003 and 32470628).

摘　　要：Trimethylation of histone H3K4(H3K4me3)is widely distributed at numerous actively transcribed protein-coding genes throughout the genome.However,the interplay between H3K4me3 and other chromatin modifications in plants remains poorly understood.In this study,we show that the Arabidopsis thaliana ALFIN-LIKE(AL)proteins contain a C-terminal PHD finger capable of binding to H3K4me3 and a PHD-associated AL(PAL)domain that interacts with components of the Polycomb repressive complex 1,thereby facilitating H2A ubiquitination(H2Aub)at H3K4me3-enriched genes throughout the genome.Furthermore,we demonstrate that loss of function of SDG2,encoding a key histone H3K4 methyltransferase,leads to a reduction in H3K4me3 level,which subsequently causes a genome-wide decrease in H2Aub,revealing a strong association between H3K4me3 and H2Aub.Finally,we discover that the PAL domain of AL proteins interacts with various other chromatin-related proteins or complexes,including those involved in regulating H2A.Z deposition,H3K27me3 demethylation,histone deacetylation,and chromatin accessibility.Our genome-wide analysis suggests that the AL proteins play a crucial role in coordinating H3K4me3 with multiple other chromatin modifications across the genome.

关 键 词：ALFIN-like proteins PRC1 HISTONE H3K4me3 H2Aub H2A.Z 

分 类 号：Q94[生物学—植物学]