延长盐单胞菌生产依克多因高密度发酵工艺优化  

High-Density Fermentation Process Optimization for Ectoine production by Halomonas elongata DSM 2581^(T)

作　　者：张悦[1] 刘昊 刘玮 余君雄 肖扬 郇晶伟 颜诚旭 庄英萍[1] 王泽建[1] ZHANG Yue;LIU Hao;LIU Wei;YU Junxiong;XIAO Yang;XUN Jingwei;YAN Chengxu;ZHUANG Yingping;WANG Zejian(School of Biotechnology,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学生物工程学院,上海200237

出　　处：《华东理工大学学报(自然科学版)》2025年第1期50-59,共10页Journal of East China University of Science and Technology

基　　金：国家重大专项项目(2020YFA096800)。

摘　　要：建立了延长盐单胞菌(Halomonas elongata DSM 2581T)高效合成依克多因的高密度发酵工艺。通过单因素实验、Plackett-Burman实验与Central Composite Design系统考察了合成培养基营养组分对H.elongata DSM 2581T发酵代谢的影响。优化结果表明:3 g/L柠檬酸钠,3 g/L氯化铵,80 g/L氯化钠的培养基组分能够显著促进H.elongata DSM 2581T菌体的生长和产物合成。根据H.elongata DSM 2581T在5 L发酵罐中优化后的培养基条件下的生长代谢情况,建立了葡萄糖连续流加和pH反馈控制(氨水)的补料方式进行H.elongata DSM 2581T高密度发酵工艺,实施了将对数生长期发酵液中氯化钠质量浓度从80 g/L提高到130 g/L的快速盐梯度冲击策略,发酵13 h后OD600最高达到169.3,依克多因产量和合成速率分别达到20 g/L和1.54 g/(L·h)。这项研究为实现依克多因的低成本、高效率工业化生产提供了理论指导。In this study,a high-density fermentation process of Halomonas elongate DSM 2581^(T) was established,which had the advantage of high ectoine production rate.The effects of nutrient components of defined medium on fermentation and metabolism of H.elongata DSM 2581^(T) were investigated by single factor experiment,Plackett-Burman and Central Composite Design.The results showed that sodium citrate(3 g/L),NH_(4)Cl(3 g/L)and NaCl(80 g/L)could significantly promote the growth and ectoine production of H.elongata DSM 2581^(T).Furthermore,according to the growth and metabolism of H.elongata in the defined medium in a 5 L fermentor,the feeding mode of glucose continuous flow plus and pH feedback control(ammonia water)was established to carry out the high-density fermentation process of H.elongata DSM 2581^(T).Salt shock,which increased the NaCl concentration in the fermentation broth from 80 g/L to 130 g/L in the exponential growth phase,resulted in OD_(600) reaching 169.3 after 13 h of fermentation,with ectoine production and ectoine synthesis reaching 20 g/L and 1.54 g/(L·h),respectively.This study provides theoretical guidance for the low-cost and efficient industrial ectoine production.

关 键 词：延长盐单胞菌 合成培养基 高细胞密度培养 依克多因 盐冲击 

分 类 号：Q815[生物学—生物工程]