基于PI3K/Akt信号通路探讨电针改善FD模型大鼠胃动力的机制  

Study on Mechanism of Electroacupuncture Improving Gastric Motility in FD Model Rats Based on PI3K/Akt Signaling Pathway

作　　者：尚冉冉 周丽 张红星 Shang Ranran;Zhou Li;Zhang Hongxing(Department of Orthopedics,Wuhan Integrated Traditional Chinese and Western Medicine Hospital,Wuhan 430022,China;Department of Rehabilitation Medicine,Wuhan Integrated Traditional Chinese and Western Medicine Hospital,Wuhan 430022,China;College of Traditional Chinese Medicine,School of Medicine,Jianghan University,Wuhan 430056,China;Institute of Acupuncture and Moxibustion,Jianghan University,Wuhan 430056,China)

机构地区：[1]武汉市中西医结合医院骨科,武汉430022 [2]武汉市中西医结合医院康复医学科,武汉430022 [3]江汉大学医学部中医学院,武汉430056 [4]江汉大学针灸研究所,武汉430056

出　　处：《华中科技大学学报(医学版)》2025年第1期67-72,共6页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：武汉市卫生健康科研基金资助项目(No.WZ22Q34)。

摘　　要：目的探讨电针通过PI3K/Akt信号通路调控肠神经系统改善功能性消化不良(functional dyspepsia,FD)模型大鼠胃动力障碍的机制。方法采用SPF级SD大鼠随机分为空白组、模型组、电针组。模型组和电针组采用多因素应激干预法构建FD大鼠模型,造模周期为14 d。造模成功后电针组予以电针足三里和太冲穴进行干预,干预周期为10 d。分别于实验开始时(0 d)、造模结束后(14 d)、电针干预结束后(24 d),3次测量大鼠体重及日饮食量;干预结束后测量各组大鼠胃内残留率、胃窦组织形态结构、胃窦组织内PI3K、Akt、GFAP蛋白表达水平及部位。结果(1)造模前,各组大鼠体重之间的差异无统计学意义(均P>0.05);电针干预后,各组大鼠体重为空白组>电针组>模型组,组间差异具有统计学意义(均P<0.01)。(2)造模前,各组大鼠饮食量之间的差异无统计学意义(均P>0.05);电针干预后,各组大鼠饮食量为空白组>电针组>模型组,组间差异均具有统计学意义(均P<0.01)。(3)各组大鼠胃内残留率为空白组<电针组<模型组,组间差异均具有统计学意义(均P<0.05)。(4)各组大鼠胃窦组织未见出血、水肿、溃疡等器质性病变。(5)各组大鼠胃窦组织内PI3K、Akt蛋白表达水平为空白组>电针组>模型组,组间差异具有统计学意义(均P<0.05);且PI3K、Akt蛋白均表达在胃窦组织肌层内。(6)各组大鼠胃窦组织内肠神经胶质细胞(enteric glial cell,EGC)数量为空白组>电针组>模型组,组间差异具有统计学意义(均P<0.05)。结论电针能够改善FD模型大鼠胃动力的机制可能为电针能够激活胃窦组织肌层内PI3K/Akt信号通路,上调EGC数量,改善肠神经系统功能。Objective To investigate the mechanism of electroacupuncture to improve gastric motility in functional dyspepsia(FD)model rats by regulating enteric nervous system through PI3K/Akt signaling pathway.Methods SPF grade SD rats were used and randomly divided into blank group,model group,and electroacupuncture group.The multifactorial stress intervention method was used in model group and electroacupuncture group to construct the FD rat model,and the modeling period was 14 days.After successful modeling,the electroacupuncture group was treated with electroacupuncture on the ST36 and LR3 for 10 days.The body weight and food intake of the rats were measured three times at the beginning of the experiment(0 d),at the end of the modeling period(14 d),and at the end of the electroacupuncture intervention(24 d),respectively.At the end of the intervention,the intragastric residual rate,the morphological structure of gastric antrum,and the protein expression levels and locations of PI3K,Akt,and GFAP in the gastric antrum tissue were measured in each group of rats.Results(1)Before modeling,the difference between the body weight of rats in each group was not statistically significant(all P>0.05).After electroacupuncture intervention,the body weight ranking of rats in each group was blank group>electroacupuncture group>model group,and the differences between groups were all statistically significant(all P<0.01).(2)Before modeling,the difference between the body intake of rats in each group was not statistically significant(all P>0.05).After electroacupuncture intervention,the ranking of food intake of rats in each group was blank group>electroacupuncture group>model group,and the differences between groups were all statistically significant(all P<0.01).(3)The ranking of intragastric residual rate of rats in each group was blank group the electroacupuncture group>the model group,and the differences between the groups were all statistically significant(all P<0.05).PI3K and Akt proteins were all expressed in the muscular layer of g

关 键 词：电针 功能性消化不良 肠神经系统 肠神经胶质细胞 胃动力 

分 类 号：R573[医药卫生—消化系统]