炎症微环境下miR-210调控Twist1对牙周膜干细胞成骨分化的影响  

The effects of miR-210 on osteogenic differentiation of periodontal ligament stem cells via regulating Twist1 in inflammatory microenvironment

作　　者：郑兴 何家才[2] ZHENG Xing;HE Jiacai(Department of Stomatology,Huainan Yangguang Xinkang Hospital,Anhui Province,China,232000;Dental Implant Center Stomatological College&Hospital Anhui Medical University,Hefei)

机构地区：[1]淮南阳光新康医院口腔科,232000 [2]安徽医科大学口腔医学院,安徽医科大学附属口腔医院

出　　处：《实用口腔医学杂志》2025年第1期21-25,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81771117)。

摘　　要：目的:研究炎症微环境下微小RNA(miR)-210调控Twist1对牙周膜干细胞(PDLSCs)成骨分化的影响。方法:培养PDLSCs,分为对照组、炎症组、miR-阴性对照(NC)组、炎症+miR-NC组、炎症+miR-210组、miR-NC+NC质粒组、炎症+miR-NC+NC质粒组、炎症+miR-210+NC质粒组、炎症+miR-210+Twist1质粒组,各炎症组均采用10μg/L肿瘤坏死因子-α处理的方式建立炎症微环境,采用脂质体转染miR序列或质粒。处理24 h后检测miR-210和Twist1的表达水平;成骨诱导分化2周后检测茜素红染色的A590水平及Runt相关转录因子2(Runx2)、骨钙素(OCN)的表达水平。结果:炎症组的miR-210表达水平低于对照组,Twist1表达水平高于对照组(P<0.05);炎症+miR-NC组的miR-210表达水平及成骨诱导后的A590水平、Runx2及OCN表达水平均低于miR-NC组,Twist1表达水平高于miR-NC组(P<0.05);炎症+miR-210组的miR-210表达水平及成骨诱导后的A590水平、Runx2及OCN表达水平均高于炎症+miR-NC组,Twist1表达水平低于炎症+miR-NC组(P<0.05);miR-210组的Twist1表达水平及野生型Twist1报告基因的荧光值均低于miR-NC组(P<0.05);炎症+miR-210+Twist1质粒组的Twist1表达水平高于炎症+miR-210+NC质粒组,成骨诱导后的A590水平、Runx2及OCN表达水平均低于炎症+miR-210+NC质粒组(P<0.05)。结论:炎症微环境下miR-210表达降低,进而增加Twist1表达并抑制PDLSCs成骨分化。Objective:To investigate the effects of microRNA-210(miR-210)on osteogenic differentiation of periodontal ligament stem cells(PDLSCs)via regulating Twist1 in inflammatory microenvironment.Methods:PDLSCs were cultured and divided into control group,inflammation group,miR-negative control(NC)group,inflammation+miR-NC group,inflammation+miR-210 group,miR-NC+NC plasmid group,inflammation+miR-210+NC plasmid group and inflammation+miR-210+Twist1 plasmid group.The inflammation microenvironment was established by 10μg/L TNF-αtreatment in the inflammation groups,and the miR sequences or plasmids were transfected by liposomes.The expression levels of miR-210 and Twist1 were detected after 24 h treatment.A590 levels of alizarin red staining and expression levels of Runt-related transcription factor 2(Runx2)and osteocalcin(OCN)detection were performed after 2 weeks of osteogenic differentiation induction.Results:The expression level of miR-210 in inflammation group was lower and the expression level of Twist1 was higher than that in control group(P<0.05).The expression level of miR-210 and A590,Runx2 and OCN after osteogenic induction in the inflammation+miR-NC group was lower than that in the miR-NC group,and the expression level of Twist1 was higher than that in the miR-NC group(P<0.05).The expression level of miR-210,A590,Runx2 and OCN after osteogenic induction in the inflammation+miR-210 group was higher than those in inflammation+miR-NC group,and the expression level of Twist1 was lower than that in inflammation+miR-NC group(P<0.05).The expression level of Twist1 and the fluorescence value of wild-type Twist1 reporter gene in miR-210 group was lower than those in miR-NC group(P<0.05).The expression level of Twist1 in the inflammation+miR-210+Twist1 plasmid group was higher than that in the inflammation+miR-210+NC plasmid group,and the level of A590,Runx2 and OCN after osteogenesis induction were lower than that in the inflammation+miR-210+NC plasmid group(P<0.05).Conclusion:The miR-210 expression decreace followed by

关 键 词：牙周炎 牙周膜干细胞 MIR-210 TWIST1 成骨分化 

分 类 号：R781.3[医药卫生—口腔医学]