基于数据挖掘和网络药理学探讨口疮治疗的中医用药规律及潜在作用机制  

The medication patterns and potential mechanisms of Chinese medicine in the treatment of oral ulcers studied by data mining and network pharmacology

作　　者：易园园 张平[1] 袁军[2] YI Yuanyuan;ZHANG Ping;YUAN Jun(College of Traditional Chinese Medicine,Hubei University of Traditional Chinese Medicine,China,430065 Wuhan;Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan)

机构地区：[1]湖北中医药大学中医学院,武汉430065 [2]湖北省中医院

出　　处：《实用口腔医学杂志》2025年第1期52-59,共8页Journal of Practical Stomatology

基　　金：国家自然科学基金项目(编号:82074364);湖北省自然科学基金创新发展联合基金(项目:2024AFD293)。

摘　　要：目的:挖掘中医药治疗口疮组方用药规律,并探讨核心药物组合作用机制。方法:检索并筛选中国知网(CNKI)、维普中文期刊(VIP)、万方数据(Wanfang)以及中国生物医学文献(CBM)中相关文献,运用EXCEL软件建立中药复方治疗口疮的数据库,并对筛选的中药进行频次统计、关联规则及聚类分析。通过TCMSP、GeneCards、STRING、DAVID数据库及Cytoscape软件分析药物治疗口疮的活性成分、靶点以及相关通路。结果:共有548篇文献符合筛选条件,涉及中药368味,发现超过100次的高频药物有34味药,以清热药、补虚药居多;药物以寒、苦为主;归经以肝经、肺经较多。进一步分析得到支持度和置信度最高的核心药物组合为“甘草-黄芩-黄连”;对“甘草-黄芩-黄连”进行网络药理学分析,发现其主要活性成分为槲皮素、山奈酚、汉黄芩素等,AKT1、IL6、TNF、TP53、IL1β、JUN等为其关键核心靶点;KEGG通路富集分析发现其主要涉及癌症信号通路、NF-κB信号通路、PI3K-Akt信号通路、MAPK信号通路、JAK-STAT信号通路等。结论:中医药治疗口疮处方用药多以益气和胃、辛开苦降为主,“甘草-黄芩-黄连”药对配伍治疗口疮具有重要意义。Objective:To explore the prescription and medication patterns of traditional Chinese medicine for treating oral ulcers,and to explore the mechanism of the action of core drug combinations.Methods:The relevant literature in China Knowledge Network(CNKI),Wipro Chinese Journals(VIP),Wanfang Data(Wanfang)and Chinese Biomedical Literature(CBM)were searched and screened,EXCEL software was used to establish a database of traditional Chinese medicines compounded for the treatment of aphthous stomatitis,the screened traditional Chinese medicines were analyzed in terms of frequency statistics,association rules,and studied by clustering analysis.The active ingredients,targets,and related pathways of core drug combinations for treating oral ulcers were studied by TCMSP,GeneCards,STRING,DAVID databases and Cytoscape software.Results:A total of 548 articles met the screening criteria,involving 368 traditional Chinese medicines.34 high-frequency drugs were found in more than 100 studies,with the majority of heat clearing and deficiency tonifying drugs.The drugs were mainly cold and bitter,the meridians of the liver and lung were more common.Further analysis revealed that the core drug combination with the highest support and confidence was“licorice-baicalin-huanglian”.Network pharmacology analysis of“licorice-baicalin-huanglian”revealed that its main active ingredients were quercetin,kaempferol and wogonin,while AKT1,IL6,TNF,TP53,and IL1β,JUN and others were the key core targets.KEGG pathway enrichment analysis revealed that it mainly involved cancer signaling pathway,NF-κB,PI3K-Akt,MAPK and JAKSTAT signaling pathway,etc.Conclusion:The prescription of traditional Chinese medicine for treating oral ulcers mainly focuses on tonifying qi and stomach,relieving pain and lowering bitterness.The combination of“licorice-huangqin-huanglian”medicine is important in the treatment of oral ulcers.

关 键 词：口疮 数据挖掘 网络药理学 用药规律 作用机制 

分 类 号：R781.5[医药卫生—口腔医学]