基于高通量测序技术对口腔扁平苔藓患者相关癌变差异LncRNAs的筛选与分析  

Screening and analysis of cancer-related differences of LncRNAs in patients with oral lichen planus based on high throughput sequencing technology

作　　者：董文亮[1] 黄一丹 郭文卓 杨慧霞 张敬[1] DONG Wenliang;HUANG Yidan;GUO Wenzhuo;YANG Huixia;ZHANG Jing(Stomatology Hospital,General Hospital of Ningxia Medical University,China,750004 Yinchuan;Stomatology College,Ningxia Medical University,Yinchuan)

机构地区：[1]宁夏医科大学总医院口腔医院牙体牙髓病科,银川750004 [2]宁夏医科大学口腔医学院口腔内科学系

出　　处：《实用口腔医学杂志》2025年第1期80-87,共8页Journal of Practical Stomatology

基　　金：宁夏自然科学基金项目(编号:2020AAC03359);宁夏医科大学校级科研项目(编号:XZ2023018);宁夏医科大学校级科研项目(编号:XM2019107);中华口腔医学会西部行口腔医学临床科研基金项目(编号:CSA-W2017-02)。

摘　　要：目的:筛选并分析口腔扁平苔藓(OLP)黏膜组织的长链非编码RNAs(LncRNAs)的癌变相关差异性表达谱,并对其功能进行初步分析,探索其在OLP发生发展过程中可能的作用。方法:收集OLP(糜烂型)病损黏膜组织和正常黏膜组织各5例,应用高通量测序技术构建OLP中基因差异表达谱,生物信息学分析得到与口腔扁平苔藓癌变关系密切的LncRNAs。结果:筛选获得400个与OLP相关的LncRNAs,其中250个表达上调,150个表达下调,得到5个与口腔扁平苔藓癌变相关的差异表达LncRNAs:54055、100128560、399717、378825、100130231。结论:筛选得到的OLP病损黏膜中差异表达LncRNAs400个,发现其中5个LncRNAs可能与OLP发病、癌变倾向相关。Objective:To screen and analyze the carcinogenesis-related differential expression profile of long non-coding RNAs(LncRNAs)in oral lichen planus(OLP)mucosa tissue,and preliminarily analyze their functions,to explore their possible role in the development of OLP.Methods:High-throughput sequencing technology was used to construct differential expression profile from 5 cases of erosive OLP lesions and 5 of normal oral mucosa.LncRNAs that are closely related to the carcinogenesis of OLP were obtained by bioinformatics analysis.Results:400 LncRNAs associated with OLP were screened,of which 250 were up-regulated and 150 were down-regulated,and 5 LncRNAs were obtained with differential expression associated with OLP carcinogenesis:LncRNA 54055,100128560,399717,378825 and 100130231.Conclusion:400 LncRNAs are differentially expressed in the mucosa of erosive OLP lesions,and 5 of them are related to the incidence and carcinogenesis tendency of OLP.

关 键 词：长链非编码RNAs 口腔扁平苔藓 高通量测序 生物信息学分析 癌变 

分 类 号：R781.5[医药卫生—口腔医学]