持续性根尖周炎患牙根尖区微生物群落分析研究  

Microbial community of root apical region with persistent apical periodontitis

作　　者：孙晓强[1] 杨子 聂勇[2] 侯本祥[3] SUN Xiaoqiang;YANG Zi;NIE Yong;HOU Benxiang(Department of Endodontics,Capital Medical University School of Stomatology,China,100069 Beijing;College of Engineering,Peking University,Beijing;Center for Microscope Enhanced Dentistry,Capital Medical University School of Stomatology,Beijing)

机构地区：[1]首都医科大学附属北京口腔医院牙体牙髓科,北京100069 [2]北京大学工学院 [3]首都医科大学附属北京口腔医院口腔显微诊疗中心

出　　处：《实用口腔医学杂志》2025年第1期93-97,共5页Journal of Practical Stomatology

基　　金：北京市东城区科技计划项目(编号:2017-3-004)。

摘　　要：目的:通过高通量测序进行持续性根尖周炎患牙根尖段微生物群落分析。方法:纳入持续性根尖周炎患牙6例,显微根尖手术切除根尖3 mm组织,提取细菌基因组DNA,选择可变区的特定区段(V3-V4)进行聚合酶链反应扩增,随后进行Illumina高通量测序。结果:6例样本总检出447个操作分类单元(OTU),平均OTU数为(164.83±56.24),以厚壁菌门、放线菌门、拟杆菌门、变形菌门和梭杆菌门为优势菌门,以双歧杆菌、链球菌、芽孢杆菌、肠球菌、卟啉单胞菌为核心菌属。通过层次聚类分析可将6个样本分为A和B两组,A组以普雷沃氏菌及密螺旋体等为主,B组以双歧杆菌、链球菌及肠球菌为主。结论:持续性根尖周炎患牙根尖段存在多菌种组成的微生物群落,并可依据细菌构成可分为不同模式,菌种间存在相互依存关系,可能为对抗持续性感染提供新策略。Objective:To characterize the bacterial communities present in the root apical regions associated with persistent apical periodontitis(PAP).Methods:6 periapical lesion samples were collected from the teeth with PAP.DNA was extracted and amplified by PCR based on the V3-V4 region of 16S rRNA gene.Illumina high throughput sequencing was applied to achieve the information of microbial community.Results:In total,447 OTUs were assigned,the average number of OTUs for the samples was 164.83±56.24.The phyla Firmicutes,Actinobacteria,Bacteroidetes,Proteobacteria and Fusobacteria were detected with high relative abundances in all samples.At the genus level,the prevalent bacterial genera were Bifidobacterium,Streptococcus,Bacillus,Enterococcus and Porphyromonas,which were abundantly detected in all samples.The 6 samples were divided into 2 groups by hierarchical cluster analysis.Prevotella and Treponema were abundant in group A,Bifidobacterium,Streptococcus and Enterococcus were abundant in group B.Conclusion:The bacterial profiles of root apical region of the teeth with PAP can be illustrated that diverse bacterial communities located in apical lesions.The bacterial composition of PAP can be divided into 2 microbial pattern and the relationship between different bacteria may guide new strategies to control infection.

关 键 词：持续性根尖周炎 显微根尖手术 根尖区 微生物群落 高通量测序 

分 类 号：R781.3[医药卫生—口腔医学] R780.2[医药卫生—临床医学]