鳖甲煎丸对胰腺癌细胞PANC-1增殖、凋亡和EMT的干预作用  

Intervention effects of BJJP on the proliferation,apoptosis,and EMT of PANC-1 pancreatic cancer cells

作　　者：熊亚南 李以恒 张荣花 章广玲 刘志勇 XIONG Yanan;LI Yiheng;ZHANG Ronghua;ZHANG Guangling;LIU Zhiyong(Hebei Key Laboratory for Chronic Diseases,School of Basic Medicine,North China University of Science and Technology,Tangshan 063210,China;Hebei Provincial Key Laboratory of Medical-Industrial Integration Precision Medicine,North China University of Science and Technology Affiliated Hospital,Tangshan 063000,China)

机构地区：[1]华北理工大学基础医学院,河北省慢性疾病重点实验室,河北唐山063210 [2]华北理工大学附属医院,河北省医工融合精准医疗重点实验室,河北唐山063000

出　　处：《河北大学学报(自然科学版)》2025年第1期70-81,共12页Journal of Hebei University(Natural Science Edition)

基　　金：河北省自然科学基金资助项目(H2023209047,H202409077);河北省教育厅在读研究生创新能力培养项目(CXZZSS2024056)。

摘　　要：为探讨鳖甲煎丸(biejiajian pill,BJJP)对胰腺癌细胞增殖、凋亡和上皮-间充质转化(EMT)的干预作用.采用CCK-8、集落形成、Western blot、TUNEL划痕、Transwell和免疫荧光等实验方法检测BJJP含药血清对人正常胰腺导管上皮细胞HPNE活力的影响,对胰腺癌细胞PANC-1增殖、凋亡、迁移、侵袭、EMT以及PI3K/AKT通路蛋白表达的影响.结果显示:BJJP含药血清对HPNE细胞的活力无明显影响,具有抑制PANC-1细胞的增殖、迁移、侵袭、EMT进程的能力,促进了PANC-1细胞凋亡,抑制了PI3K/AKT通路,综上,鳖甲煎丸能抑制胰腺癌PANC-1细胞的恶性生物学行为,其机制可能与调节PI3K/AKT通路有关.To investigate the effects of BJJP on the proliferation,apoptosis,and EMT of PANC-1 pancreatic cancer cells.CCK-8,Colony formation,Western blot,TUNEL scratch,Transwell and immunofluorescence assays were used to detect the effect of BJJP-containing serum on the activity of human normal pancreatic ductal epithelial cells HPNE,and detect the effects of BJJP-containing serum on the proliferation,apoptosis,migration,invasion,EMT,and the protein expression of PI3K/AKT pathway of pancreatic cancer cells PANC-1.The results showed that BJJP-containing serum has no significant effect on the activity of HPNE cells but inhibits the proliferation,migration,invasion,EMT process and PI3K/AKT pathway,enhances the apoptosis of pancreatic cancer PANC-1 cells.Studies have shown that BJJP inhibits the malignant biological behavior of pancreatic cancer PANC-1 cells,and the mechanism may be related to the regulation of PI3K/AKT pathway.

关 键 词：鳖甲煎丸 胰腺癌 增殖 凋亡 上皮-间充质转化 

分 类 号：R735.9[医药卫生—肿瘤]