In vivo self-assembled bispecific fluorescence probe for early detection of bladder cancer and metastasis  

活体自组装双特异性荧光探针用于膀胱癌及转移的早期检测

作　　者：Da-Yong Hou Ni-Yuan Zhang Peng Zhang Xiang-Peng Li Jiong-Cheng Wu Mei-Yu Lv Zhi-Jia Wang Xing-Jie Hu Jian-Xiao Liang Hong-Lei Wang Yue-Ze Wang Hui-Hui You Hong-Wei An Hao Wang Wanhai Xu 侯大勇;张薿元;张鹏;李翔鹏;武炯呈;吕美玉;王志佳;胡兴洁;梁剑萧;王宏磊;王悦泽;游慧慧;安红维;王浩;徐万海

机构地区：[1]NHC Key Laboratory of Molecular Probe and Targeted Theranostics,Heilongjiang Key Laboratory of Scientific Research in Urology,Harbin Medical University,Harbin 150001,China [2]Department of PET-CT/MRI,Harbin Medical University Cancer Hospital,Harbin 150001,China [3]CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,CAS Center for Excellence in Nanoscience,National Center for Nanoscience and Technology(NCNST),Beijing 100190,China

出　　处：《Science Bulletin》2025年第3期407-418,共12页科学通报(英文版)

基　　金：supported by the National Key R&D Program of China(2022YFA1205701 and 2020YFA0210800);the National Natural Science Foundation of China(52322308,51725302,52273126,11621505,and 82302266);Beijing Nova Program(20230484237);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB36000000);the Key Research and Development Program of Heilongjiang Province(2022zX06004);Hong Kong scholar project(Xj2024052);Harbin Medical University Cancer Hospital Haiyan Foundation(JYQ2024-03 and JZD2024-17);the Nn10 program of Harbin Medical University Cancer Hospital(Nn102024-01);the Climing program of Harbin Medical University Cancer Hospital(PDYS2024-03);China Postdoctoral Science Foundation(2024MD753933);Heilongjiang Postdoctoral Fund(LBH-Z23221).

摘　　要：Tumor metastasis accounts for over 90%of tumor-related deaths,prompting the development of fluorescently labeled tumor-specific molecular imaging agents for differentiating tumors from normal tissues.However,early detection of metastasis lesions by tracking tumor markers alone has proven to be challenging.Herein,we reported a glycopeptide-based bispecific fluorescence probe(bsProbe)for earlier detection of bladder cancer and metastasis.By simultaneously recognition(tumor&tumor microenvironment)and in vivo self-assembly,the tumor accumulation of bsProbe(12.3%ID/g)was obviously increased by∼6 fold compared with that in CXCR4 specific fluorescence probe(sProbe),indicating the obvious advantages of bsProbe over existing tumor metastasis detection probes.Additionally,bsProbe substantially broadens the tumor diagnosis window and enhances the detection signal to noise ratio(SNR:approximately 9.5),permitting early diagnosis of lung micro-metastasis(∼1 mm),precise identifying of tumor boundaries and micro-tumors in orthotopic tumor models.More importantly,bsProbe was demonstrated to distinguish malignant from benign specimen with a specificity of 90.48%and sensitivity of 92.22%in 195 clinical specimens of bladder cancer patients.Taken together,this novel synergetic targeting(CD206×CXCR4)strategy provides an attractive method for earlier detection of bladder cancer and metastasis,which might be further extended to the imaging-guided surgery of clinical invisible tumors.

关 键 词：Self-assembly PEPTIDE NANOMATERIAL Fluorescence imaging Bladder cancer 

分 类 号：R73[医药卫生—肿瘤]