CCAAT增强子结合蛋白β在胃癌组织中的表达及其临床意义  

Expression and clinical significance of the CCAAT enhancer-binding proteinβin gastric cancer tissues

作　　者：钟海均[1] 吕汪霞[1] 凌哲南 李龙 杜侠莹 王文静 卜凡 范承源 凌志强[6] Zhong Haijun;Lyu Wangxia;Ling Zhenan;Li Long;Du Xiaying;Wang Wenjing;Bu Fan;Fan Chengyuan;Ling Zhiqiang(Department of Oncology,Zhejiang Cancer Hospital,Hangzhou 310022,China;General Department of Surgery,First Affiliated Hospital of Zhejiang University,Hangzhou 310000,China;Postgraduate training base Alliance of Wenzhou Medical University(Zhejiang Cancer Hospital),Hangzhou Institute of Medicine,Chinese Academy of Sciences,Hangzhou 310022,China;Zhejiang Cancer Hospital,Hangzhou 310022,China;Department of Pathophysiology,School of Basic Medicine,Zhengzhou University,Zhengzhou 450001,China;Zhejiang Second Clinical College of TCM University,Hangzhou 310053,China)

机构地区：[1]浙江省肿瘤医院肿瘤内科,杭州310022 [2]浙江大学附属第一医院普外科,杭州310000 [3]温州医科大学研究生培养基地(浙江省肿瘤医院),中国科学院杭州医学研究所,杭州310022 [4]浙江中医药大学第二临床学院,杭州310053 [5]郑州大学基础医学院病理生理学系,郑州450001 [6]浙江省肿瘤医院浙江省肿瘤研究所,杭州310022

出　　处：《中华实验外科杂志》2024年第12期2829-2833,共5页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金面上项目(32271238);国家卫生健康委科学研究基金-浙江省卫生健康重大科技计划重点项目(WKJ-ZJ-2117);浙江省自然科学基金面上项目(LY20H160005)。

摘　　要：目的观察CCAAT增强子结合蛋白β(C/EBPβ)在胃癌组织中的表达并探讨其临床意义。方法 2021年1月至2023年12月期间在浙江省肿瘤医院收集100例胃癌患者的肿瘤及其配对癌旁正常组织, 采用免疫组织化学和Real-time RT-PCR检测标本中C/EBPβ蛋白和mRNA表达水平, 并分析C/EBPβ表达与患者临床病理参数之间的相关性及对患者预后的影响。计量资料采用Personχ^(2)检验分析。结果在胃癌组织中C/EBPβ蛋白和mRNA的高表达率分别为59.0%(59/100)、69.0%(69/100), 显著高于癌旁正常组织的高表达率[13.0%(13/100)、3.0%(3/100)]。胃癌组织中C/EBPβ蛋白表达水平与肿瘤大小(67.74%比42.11%)、肿瘤部位(52.87%比92.31%)、浸润深度(10.53%比69.14%)、淋巴管侵犯(0.00%比61.70%)、淋巴结转移(0.00%比68.24%)、远处转移(55.79%比100.00%)及TNM分期(4.17%比75.00%)均呈显著负相关(χ^(2)=11.833、4.665、21.653、9.140、9.140、3.690、39.328, P<0.05);而与性别(59.72%比53.57%)、肿瘤诊断时年龄(65.52%比54.93%)、肿瘤分化(56.36%比60.00%)、静脉侵犯(52.63%比61.29%)均无明显相关(χ^(2)=0.395、0.940、0.134、0.792, P>0.05)。胃癌组织中C/EBPβ mRNA表达水平与患者临床病理参数之间的相关性分析显示相同的趋势。胃癌组织中C/EBPβ蛋白表达水平与患者预后无显著相关, 两者中位OS差异无统计学意义(中位OS:15个月比15个月)(χ^(2)=48.439, P<0.05)胃癌组织中C/EBPβ mRNA表达水平与患者预后无显著相关, 两者中位OS差异无统计学意义(中位OS:16个月比16个月)(χ^(2)=53.175, P<0.05)。Cox单因素回归分析表明肿瘤浸润深度(HR=2.054, 95%CI:1.188~3.552)、淋巴结转移(HR=2.408, 95%CI:0.966~6.003)、远处转移(HR=2.663, 95%CI:1.063~6.671)及TNM分期(HR=2.344, 95%CI:1.394~3.941)都与胃癌患者的预后显著相关(P<0.05), 但Cox多因素回归分析发现肿瘤浸润深度(HR=1.404, 95%CI:0.691~2.855)、淋巴结转移(HR=1.518, 95%CI:0.542~4.256)、远处转移(HR=ObjectiveTo observe the expression of the CCAAT enhancer-binding proteinβ(C/EBPβ)in gastric cancer tissues and explore its clinical significance.MethodsFrom January 2021 to December 2023,the tumors and their paired adjacent normal tissues from 100 gastric cancer patients were collected in Zhejiang Cancer Hospital,and the expression levels of C/EBPβprotein and mRNA in the specimens were measured by immunohistochemistry and real-time RT-PCR,and the correlation between C/EBPβexpression and the clinicopathological parameters and the impact on patient prognosis were analyzed.Measurement data were analyzed by Personχ^(2) test.ResultsThe high expression rate of C/EBPβprotein and mRNA were 59.0%(59/100)and 69.0%(69/100),respectively,which were significantly higher than those in the adjacent normal tissues(13.0%,13/100;3.0%,3/100).The expression level of C/EBPβprotein in gastric cancer tissues was correlated with tumor size(67.74%vs.42.11%),tumor site(distal vs.cardia)(52.87%vs.92.31%),the depth of infiltration(10.53%vs.69.14%),lymphatic vessel invasion(0.00%vs.61.70%),lymph node metastasis(0.00%vs.68.24%),distant metastasis(55.79%vs.100.00%)and TNM stage(4.17%vs.75.00%)(χ^(2)=11.833,4.665,21.653,9.140,9.140,3.690,39.328,P<0.05);but had no significant correlation with gender(59.72%vs.53.57%),age at tumor diagnosis(65.52%vs.54.93%),tumour differentiation(56.36%vs.60.00%)and venous invasion(52.63%vs.61.29%)(χ^(2)=0.395,0.940,0.134,0.792,P>0.05).Correlation analysis between C/EBPβmRNA expression levels in GC tissues and clinicopathological parameters of patients showed the same trend.The expression level of C/EBPβprotein in gastric cancer tissue was not significantly related to patient prognosis,there was no significant difference in median OS between them both(median OS:15 months vs.15 months)(χ^(2)=48.439,P<0.05).The expression level of C/EBPβmRNA in gastric cancer tissue was not significantly related to patient prognosis,there was no significant difference in median OS between them both(median OS:16 months vs

关 键 词：胃癌 CCAAT增强子结合蛋白β 肿瘤浸润深度 转移 预后 

分 类 号：R735.2[医药卫生—肿瘤]