Alkali-extracted polysaccharides from mung bean skin possess protective effect on H_(2)O_(2)-induced IEC-6 cells:the role of NF-κB and MAPK signaling pathways  

作　　者：Shuai Chen Liuming Xie Pingwei Wen Qiang Yu Yi Chen Hanyu Lu Ting Chen Xin Tao Jianhua Xie 

机构地区：[1]State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang 330047,China [2]International Institute of Food Innovation Co.,Ltd.,Nanchang University,Nanchang 330200,China

出　　处：《Food Science and Human Wellness》2025年第1期101-111,共11页食品科学与人类健康(英文)

基　　金：financially supported by the National Key Research and Development Program of China(2023YFF1104001);Natural Science Foundation of Jiangxi Province,China(20232BCD44003);National High-Level Talents Special Support Program of China(202212)。

摘　　要：Intestinal oxidation-reduction balance is essential to protect host health.Research on the underlying mechanisms of oxidation homeostasis can provide conditions to the treatment or protection of intestinal oxidative stress.Therefore,the mechanism of polysaccharide(MBP)with alkali-extracted from mung bean skin in alleviating oxidative damage induced by H_(2)O_(2)-induced in intestinal epithelial cells(IEC)-6 cells was explored.Results indicated that MBP effectively mitigated the H_(2)O_(2)-induced decrease in IEC-6 cell viability,restored superoxide dismutase(SOD)activity,lowered malondialdehyde(MDA)content,and decreased reactive oxygen species(ROS)level.Western blotting showed that MBP may mitigate oxidative damage through NF-κB,MAPK and Nrf2 signaling pathway,and RNA sequencing(RNA-seq)analysis confirmed that MBP can protect cells by regulating MAPK and NF-κB signaling pathways.Moreover,the results of the inhibitor experiment also support the above hypothesis.These results verified that MBP has the capacity to mitigate oxidative damage induced by H_(2)O_(2)in IEC-6 cells through MAPK and NF-κB signaling pathway.The study showed that MBP can assist preventing intestinal diseases relating to oxidative stress,which could facilitate the development of functional foods.

关 键 词：POLYSACCHARIDE Alkali-extracted Oxidative stress RNA sequencing Molecular mechanism 

分 类 号：TS201.4[轻工技术与工程—食品科学] R574[轻工技术与工程—食品科学与工程]