Hyperspectral imaging for one-step growth simulation of Brochothrix thermosphacta in chilled beef during storage  

作　　者：Xiaohua Liu Binjing Zhou Jin Song Kang Tu Jing Peng Weijie Lan Jing Xu Jie Wu Juqing Wu Leiqing Pan 

机构地区：[1]College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China [2]College of Artifi cial Intelligence,Nanjing Agricultural University,Nanjing 210095,China [3]School of Food and Biological Engineering,Bengbu University,Bengbu 233030,China

出　　处：《Food Science and Human Wellness》2025年第1期226-235,共10页食品科学与人类健康(英文)

基　　金：supported by Key Research&Development Program of Jiangsu Province in China(BE2020693);Major Project of Science and Technology of Anhui Province(201903a06020010);Joint Key Project of Science and Technology Innovation of Yangtze River Delta in Anhui Province(202004g01020009);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。

摘　　要：In this work,one-step growth models using hyperspectral imaging(HSI)(400-1000 nm)were successfully developed in order to estimate the microbial loads,minimum growth temperature(T_(min))and maximum specific growth rate(μ_(max))of Brochothrix thermosphacta in chilled beef at isothermal temperatures(4-25℃).Three different methods were compared for model development,particularly using(Model Ⅰ)the predicted microbial loads from partial least squares regression of the whole spectral variables;(Model Ⅱ)the selected spectral variables related to microbial loads;and(Model Ⅲ)the first principal scores of HSI spectra by principal component analysis.Consequently,Model Ⅰ showed the best ability to predict the microbial loads of B.thermosphacta,with the coefficient of determination(R_(v)^(2))and root mean square error in internal validation(RMSEV)of 0.921 and 0.498(lg(CFU/g)).The T_(min)(-12.32℃)andμmax can be well estimated with R^(2) and root mean square error(RMSE)of 0.971 and 0.276(lg(CFU/g)),respectively.The upward trend ofμmax with temperature was similar to that of the plate count method.HSI technique thus can be used as a simple method for one-step growth simulation of B.thermosphacta in chilled beef during storage.

关 键 词：Brochothrix thermosphacta BEEF Hyperspectral imaging Growth simulation One-step analysis Predictive microbiology 

分 类 号：TS251.52[轻工技术与工程—农产品加工及贮藏工程] O657.3[轻工技术与工程—食品科学与工程]