2'-Fucosyllactose modulates the function of intestinal microbiota to reduce intestinal permeability in mice colonized by feces from healthy infants  

作　　者：Qingxue Chen Liu Yang Fangqin Xiang Ignatius Man-Yau Szeto Yalu Yan Biao Liu Jinju Cheng Lu Liu Bailiang Li Sufang Duan 

机构地区：[1]Key Laboratory of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030,China [2]College of Food Science,Northeast Agricultural University,Harbin 150030,China [3]National Center of Technology Innovation for Dairy,Hohhot 010110,China [4]Inner Mongolia Yili Industrial Group,Co.,Ltd.,Yili Maternal and Infant Nutrition Institute(YMINI),Beijing 100070,China

出　　处：《Food Science and Human Wellness》2025年第1期282-292,共11页食品科学与人类健康(英文)

基　　金：financially supported by the National Key Research and Development Program of China(2022YFF1100402);National Center of Technology Innovation for Dairy(2022-Open subject-11);Young Elite Scientist Sponsorship Program by CAST(YESS20200271);the National Natural Science Foundation of China(32101919)。

摘　　要：2'-Fucosyllactose(2'-FL)shows the potential to support intestinal health as a natural prebiotic that bridges the gap between infant formula feeding and breastfeeding.However,the effect and mechanism of 2'-FL in improving intestinal permeability are not clear.In this study,we constructed human microbiota-associated(HMA)mouse models by colonizing healthy infant feces in mice with antibiotic-depleted intestinal microbiota.The protective effect of 2'-FL on the intestinal permeability was explored using the HMA mouse models,and the combination of metagenomics was used to analyze the possible mechanisms by which the microorganisms reduced the intestinal permeability.The results showed that 2'-FL decreased the concentration of markers of intestinal permeability(enterotoxin and diamine oxidase(DAO))and increased the expression levels of tight junctions(occludin and claudin).Metagenomics revealed the enrichment of Bifidobacterium and increased the expression of glycoside hydrolases(GHs),including GH31,GH28,and GH5.In conclusion,2'-FL strengthened intestinal permeability function by improving microbiota composition to control the translocation of harmful substance.

关 键 词：2’-Fucosyllactose Intestinal permeability Intestinal microbiota BIFIDOBACTERIUM 

分 类 号：TS201.4[轻工技术与工程—食品科学] R174.4[轻工技术与工程—食品科学与工程]