Bifidobacterium longum subsp.infantis CCFM1269 promotes intestinal barrier and release of IFN-β through TLR4-TRIF dependent way in growing mice  

作　　者：Mengfan Ding Bo Yang Bowen Li Haiqin Chen Renqiang Yu RPaul Ross Catherine Stanton Shilong Jiang Jianxin Zhao Wei Chen 

机构地区：[1]State Key Laboratory of Food Science and Resources,Jiangnan University,Wuxi 214122,China [2]School of Food Science and Technology,Jiangnan University,Wuxi 214122,China [3]International Joint Research Center for Probiotics&Gut Health,Jiangnan University,Wuxi 214122,China [4]Department of Neonatology,Wuxi Maternity and Child Health Care Hospital,Affi liated Women’s Hospital of Jiangnan University,Wuxi 214122,China [5]APC Microbiome Ireland,University College Cork,Cork T12 R229,Ireland [6]Teagasc Food Research Centre,Cork P61 C996,Ireland [7]Nutrition and Metabolism Research Division,Innovation Center,Heilongjiang Feihe Dairy Co.,Ltd.,Beijing 100015,China [8]PKUHSC-China Feihe Joint Research Institute of Nutrition and Healthy Lifespan Development,Beijing 100083,China [9]National Engineering Research Center for Functional Food,Jiangnan University,Wuxi 214122,China

出　　处：《Food Science and Human Wellness》2025年第1期345-360,共16页食品科学与人类健康(英文)

基　　金：funded by the National Key R&D Program of China(2021YFD2100700);National Natural Science Foundation of China(32021005);111 project(BP0719028);Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。

摘　　要：Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to explore the effects of B.longum subsp.infantis supplementation on interferon-beta(IFN-β)secretion and intestinal barrier improvement in growing mice.Female and male mice were orally administered either saline or B.longum subsp.infantis CCFM1269 or I5TI(1×10^(9) CFU/mice per day,n=8)from 1-week-age until 3-,4-,and 5-week-age.RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2'-5'oligoadenylate synthetase(OAS).Additionally,CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway.However,this effect was not observed in 4-and 5-week-old mice.Furthermore,both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-,4-,and 5-week-old mice.In summary,the results of this study suggested that B.longum subsp.infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.

关 键 词：Bifidobacterium longum subsp.infantis Intestinal barrier interferon-beta(IFN-β)secretion Age dependence 

分 类 号：TS201.3[轻工技术与工程—食品科学]