The effects of selenium on the translation efficiency of selenoproteins GPX1,GPX4,and TXNRD1 in oxLDL-induced EA.hy926 cells  

作　　者：Jiaqi Lu Jing Meng Yanzhi Lang Abdul Rehman Yamei Yan Linwu Ran 

机构地区：[1]Key Laboratory of Environmental Factors and Chronic Disease Control,School of Public Health,Ningxia Medical University,Yinchuan 750004,China [2]School of Clinical Medicine,Ningxia Medical University,Yinchuan 750004,China [3]Goji Berry Science Research Institute,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,China

出　　处：《Food Science and Human Wellness》2025年第1期371-383,共13页食品科学与人类健康(英文)

基　　金：supported by grants from the National Natural Science Foundation of China(NSFC,81960588);the Ningxia Natural Science Foundation(2020AAC03146);support from the Ningxia Medical University。

摘　　要：Selenium is a crucial trace element that contributes to physiological processes in the body as selenoproteins.Selenoproteins serve as an integral role in the body in controlling the redox state of cells and protecting against damage induced by oxidative stress.This study aimed to investigate the effects and possible mechanism of selenium on selenoproteins expression in EA.hy926 cells induced by oxidized low density lipoprotein(oxLDL).The impact of selenium on the viability of EA.hy926 cells was detected by the methylthiazolyldiphenyltetrazolium bromide(MTT)method,and intracellular reactive oxygen species(ROS)level and mitochondrial membrane potential were assessed by fluorescent probe DCFH-DA and JC-1,respectively.RNA-seq,quantitative real-time polymerase chain reaction(qPCR),and Western blot were used to investigate the selenoprotein expression.Selenoprotein mRNA translation efficiency was analyzed by ribosome profiling(Ribo-Seq)coupled with transcriptomics.Our data showed that selenium supplementation(0.5μmol/L)significantly decreased ROS production,increased mitochondrial inner membrane potential and increased the proliferative activity of EA.hy926 cells induced by oxLDL.Moreover,The protective effects of selenium against oxLDL-induced EA.hy926 cell injury were associated with the upregulation of the expressions of selenoproteins glutathione peroxidase 1(GPX1),glutathione peroxidase 4(GPX4),and thioredoxin reductase 1(TXNRD1).Furthermore,the expressions of selenoproteins GPX1 and GPX4 were hierarchically controlled,but the expressions of selenoproteins TXNRD1 were mainly regulated by oxLDL.Finally,Ribo-Seq coupled with transcriptomics results demonstrated that the expressions of selenoproteins GPX1,GPX4,and TXNRD1 were regulated at the translation process level.These findings suggested that selenium could have preventive effects in oxLDL induced EA.hy926 cell injury by regulating the selenoprotein expression,and the selenoproteins expressions at the translation level in vascular endothelial cells need further

关 键 词：SELENIUM Oxidized low density lipoprotein(oxLDL) SELENOPROTEIN Ribosome profiling Translational efficiency 

分 类 号：TS201.4[轻工技术与工程—食品科学]