Immune checkpoint receptors and their ligands on CD8^(+)T cells and myeloma cells in extramedullary multiple myeloma  

作　　者：XIAN ZHANG ZHUANG ZHOU JUNZHE WANG MENGMENG HAN HAN LIU MEIRONG ZANG JIANNING LIU JIAPEI LU JINQIAO ZHANG GUOCHUAN ZHANG LIXIA SUN 

机构地区：[1]Department of Hematology,Third Hospital of Hebei Medical University,Shijiazhuang,050051,China [2]Department of Bone and Soft Tissue Oncology,Third Hospital of Hebei Medical University,Shijiazhuang,050051,China

出　　处：《BIOCELL》2024年第2期303-311,共9页生物细胞(英文)

基　　金：supported by S&T Program of Hebei,China(Grant Number 21377754D);the 2021 Hebei Provincial Governmentfunded Clinical Medicine Talents Training Project(Grant Number 2021-99)。

摘　　要：Background:Prognosis of multiple myeloma(MM)patients with extramedullary disease(EMD)remains poor.T cell dysfunction and an immunosuppressive environment have been reported in the bone marrow(BM)of MM patients.However,the immunosuppressive microenvironment and immune checkpoint receptors(ICRs)on CD8^(+)T cells in the EMD tissue of newly diagnosed MM(NDMM)patients have not been thoroughly studied.Methods:We investigated the expression levels of T cell immunoglobulin mucin-domain-containing-3(TIM-3)and T-cell immunoglobulin and ITIM domain(TIGIT)on CD8^(+)T cells and the expression of their ligands(Galectin-9 and CD155)on myeloma cells in EMD tissue of NDMM patients.The expression levels of TIM-3 and TIGIT on T cells and their ligands(Galectin-9 and CD155)on myeloma cells were analyzed by multicolored fluorescent flow cytometry in the peripheral blood(PB),BM,and EMD tissue.Additionally,the expression of ICRs was compared among PB,BM,and EMD tissue.Results:The expression levels of TIGIT and TIM-3 on CD8^(+)T cell subsets,including CD8^(+)Naïve T(T_(N))cells and CD8^(+)terminally differentiated effector memory T(TEMRA)cells,were significantly higher in EMD than in PB(p<0.05 for all).The expression levels of TIGIT and TIM-3 on CD8^(+)T_(N)cells were higher in EMD than in BM(p<0.05 for all).There were no differences in Galectin-9 and CD155 expression on myeloma cells among PB,BM,and EMD tissue.Conclusion:Our data add new evidence that T cells express increased TIGIT and TIM-3,suggesting that there may be T-cell exhaustion in EMD of patients with MM.

关 键 词：Multiple myeloma Extramedullary disease Immune checkpoint receptors LIGANDS T-cell exhaustion 

分 类 号：R73[医药卫生—肿瘤]