猴头菇多糖对小鼠肠道菌群的影响  

Impact of Hericium erinaceus polysaccharide on intestinal flora in mice

作　　者：吕国英[1] 王梦雨 张作法[1] LYU Guoying;WANG Mengyu;ZHANG Zuofa(Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江省农业科学院园艺研究所,浙江杭州310021

出　　处：《浙江农业学报》2024年第12期2794-2802,共9页Acta Agriculturae Zhejiangensis

基　　金：浙江省农业新品种选育重大科技专项(2021C02073);浙江省“三农九方”科技协作项目(2022SNJF047)。

摘　　要：为检验猴头菇多糖是否能改变肠道微生物菌群的多样性和丰度,评估粪便中微生物菌群变化对机体代谢的潜在影响,以成年健康小鼠为研究对象,用猴头菇多糖灌胃8 d,采集小鼠粪便,扩增粪便中微生物16S rRNA基因的V3-V4区,进行菌群群落结构和多样性比较。α多样性和β多样性分析结果表明,猴头菇多糖处理组粪便微生物具有较高的丰富度和群落差异,厚壁菌门和变形杆菌门与未用猴头菇多糖处理组对比相对丰度显著增加,而拟杆菌门的相对丰度显著降低。KEGG功能预测分析表明,粪便菌群的基因功能主要与碳水化合物代谢、脂肪代谢、辅因子和维生素的代谢等功能有关。猴头菇多糖处理组肠道微生物发酵产生的短链脂肪酸含量与未用猴头菇多糖处理组相比高出14.7%,短链脂肪酸的组成比例也有较大变化。综上可知,猴头菇多糖通过调节小鼠粪便菌群多样性影响其代谢途径,发挥对机体的调节作用。Investigate whether Hericium erinaceus polysaccharides can alter the diversity and abundance of intestinal microbial flora,and assess the potential impact of changes in fecal microbiota on metabolism,adult healthy mice were gavaged with H.erinaceus polysaccharides for 8 days,after which their feces were collected.The V3-V4 region of the 16S rRNA gene of microbes in the feces were amplified for comparison of microbial community structure and diversity.α-diversity andβ-diversity analyses showed that the fecal microbes in the H.erinaceus polysaccharide treatment group had higher richness and community differences.The relative abundance of Firmicutes and Proteobacteria significantly increased compared to the untreated group,while the relative abundance of Bacteroidetes significantly decreased;KEGG functional prediction analysis indicated that the genetic functions of the fecal microbiota were mainly related to carbohydrate metabolism,lipid metabolism,cofactor and vitamin metabolism,etc.;The short-chain fatty acids produced by the fermentation of intestinal microbes in H.erinaceus polysaccharides treatment group was 14.7%higher than those in the untreated group,and there were also significant changes in the composition ratio of short-chain fatty acids.In summary,H.erinaceus polysaccharides affect metabolic pathways by regulating the diversity of mouse fecal microbiota,playing a regulatory role in the body.

关 键 词：猴头菇多糖 16S rRNA 粪便 短链脂肪酸 

分 类 号：TS201.4[轻工技术与工程—食品科学]