刺囊酸对非小细胞肺癌PC-9细胞增殖、凋亡、迁移及侵袭的影响  

Effects of echinocystic acid on proliferation,apoptosis,migration and invasion of non-small cell lung cancer PC-9 cells

作　　者：范正媛 李亚[1,2,3] 刘素晓 李高峰[2] 李素云[1,2,3] FAN Zheng-Yuan;LI Ya;LIU Su-Xiao(Chinese Medicine Pharmacology(Respiratory)Laboratory,the First Afficiated Hospital of Henan University of Chinese Medicine,Zhengzhou 450046,Henan,China)

机构地区：[1]河南中医药大学第一附属医院中药药理(呼吸)实验室,河南郑州450046 [2]河南中医药大学河南省中医药防治呼吸病重点实验室 [3]河南中医药大学呼吸疾病中医药防治省部共建协同创新中心

出　　处：《中国老年学杂志》2025年第2期351-356,共6页Chinese Journal of Gerontology

基　　金：国家自然科学基金面上项目(82074413);河南省科技研发计划联合基金项目(222301420081);国家中医临床研究基地科研专项(2021JDZY033)。

摘　　要：目的探讨刺囊酸对非小细胞肺癌细胞增殖、凋亡、迁移及侵袭的影响及机制。方法人非小细胞肺癌PC-9细胞,分为对照组[二甲基亚砜(DMSO)溶剂对照组]、刺囊酸5、10、20μmol/L给药组,细胞计数试剂盒(CCK)-8法对细胞增殖能力进行检测;终端尿苷核苷酸末端标记(TUNEL)染色、流式细胞术对细胞凋亡能力进行检测;划痕实验对细胞迁移能力进行检测;侵袭实验对细胞侵袭能力进行检测;Western印迹法检测细胞磷脂酰肌醇-3激酶(PI3K)/蛋白激酶B(AKT)的通路磷酸化及基质金属蛋白酶(MMP)2、MMP9、B细胞淋巴瘤(Bcl)-2、Bcl-2相关X蛋白(Bax)表达。结果与对照组相比,刺囊酸5、10、20μmol/L可显著抑制细胞增殖、迁移和侵袭,明显诱导细胞凋亡,明显抑制PI3K和AKT磷酸化,明显下调MMP2和MMP9蛋白表达,明显降低Bcl-2/Bax比值(P<0.05)。结论刺囊酸能抑制PC-9细胞增殖、迁移和侵袭,诱导细胞凋亡,作用机制可能与抑制PI3K/AKT信号通路有关。Objective To explore the effects and mechanisms of echinocystic acid on the proliferation,apoptosis,migration and invasion of non-small cell lung cancer cells.Methods Human non-small cell lung cancer PC-9 cells were divided into control group[dimethyl sulfoxide(DMSO)solvent control group],and echinocystic acid 5,10 and 20μmol/L administration groups.Cell counting kit(CCK)-8 assay was used to detect the cell proliferation;TdT-mediated dUTP nick-end labeling(TUNEL)staining and flow cytometry were used to detect the cell apoptosis;cell scratch test was used to determine the cell migration;cell invasion assay was used to detect the cell invasion;Western blot was performed to detect the phosphorylation of phosphatidylinositol-3 kinase(PI3K)/protein kinase B(AKT)pathway and the expressions of matrix metalloproteinase(MMP)2,MMP9,B cell lymphoma(Bcl)-2,and Bcl-2-associated X protein(Bax).Results Compared with the control group,cell proliferation,migration and invasion were significantly inhibited,and apoptosis was significantly induced,PI3K and AKT phosphorylation were significantly inhibited,the protein expressions of MMP2 and MMP9 were significantly down-regulated,Bcl-2/Bax ratio was significantly decreased by echinocystic acid of 5,10 and 20μmol/L(P<0.05).Conclusions Echinocystic acid could inhibit the proliferation,migration and invasion,induce apoptosis of PC-9 cells,the mechanism may be related to the inhibition of PI3K/AKT signaling pathway.

关 键 词：刺囊酸 非小细胞肺癌 PC-9 磷脂酰肌醇-3激酶/蛋白激酶B 

分 类 号：R734.2[医药卫生—肿瘤]