基于特超声声流体隧道的荧光激活细胞分选技术  

Fluorescence-activated cell sorting based on ultrasonic acoustic fluid tunneling

作　　者：唐冰怡 王亚平 魏巍 段学欣 TANG Bingyi;WANG Yaping;WEI Wei;DUAN Xuexin(State Key Laboratory of Precision Measuring Technology&Instruments,College of Precision Instruments and Optoelectronics Engineering,Tianjin University,Tianjin 300072,China)

机构地区：[1]精密测试技术与仪器国家重点实验室,天津大学精密仪器与光电子工程学院,天津300072

出　　处：《分析试验室》2025年第1期22-28,共7页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(62174119)资助。

摘　　要：基于声流控技术开发了一个完整的荧光激活细胞分选(FACS)系统。该系统利用固体装配型薄膜体声波谐振器(SMR)在微流道中产生的声流体隧道,结合荧光识别激活程序,实现了对单个目标颗粒和细胞的高精度捕获、分选,具有检测和分选目标颗粒物的功能。使用该系统从非荧光聚苯乙烯微球和荧光聚苯乙烯微球的混合物中分离出荧光微球,验证该系统的稳定性。在150个/s通量情况下,纯度可以达到95%。对于细胞样本,从活死Hela细胞样品中分离出活的Hela细胞,纯度高于90%,证明了该系统在分选活死细胞中的高分辨率,并且分选前后细胞的存活率高于95%,证明了该系统的生物兼容性良好。This paper develops a complete fluorescence-activated cell sorting(FACS)system based on acoustofluidic technology.This system uses the acoustofluidic tunnel in the microfluidic channel generated by a solidly mounted film bulk acoustic wave resonator(SMR)and combines it with the fluorescence recognition activation program to achieve high-precision capture and sorting of individual target particle and cell,having the function of detecting and sorting target particles.The stability of the system was verified by separating fluorescent microspheres from a mixture of non-fluorescent and fluorescent polystyrene microspheres.The purity could reach 95%at a throughput of 150 cells/s.For cell samples,live HeLa cells were isolated from live and dead HeLa cells with a purity higher than 90%,proving the high resolution of this system in sorting live and dead cells.And the survival rates of cells before and after sorting were higher than 95%,proving the good biocompatibility of the system.

关 键 词：声流控 荧光激活细胞分选 体声波 固体装配型谐振器 

分 类 号：TH89[机械工程—仪器科学与技术]