Protective Effect of Shenfu Injection against Sepsis-induced Acute Lung Injury by Suppressing Inflammation and Apoptosis Through the Regulation of the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Pathway  

作　　者：Li-Juan Cao Hai-Tang Xie Zhong-Xia Chu Yue Ma Ming-Ming Wang Shi Zhuang 

机构地区：[1]Department of Paediatrics,Affiliated Hospital of Inner Mongolia Minzu University,Tongliao,China [2]Department of Mongolian Medicine Hand Foot Surgery,Affiliated Hospital of Inner Mongolia Minzu University,Tongliao,China

出　　处：《World Journal of Traditional Chinese Medicine》2024年第4期528-534,共7页世界中医药杂志(英文)

基　　金：supported by the Inner Mongolia University for the Nationalities General Project (NMDYB17171)

摘　　要：Objective:Sepsis-induced acute lung injury(ALI) is a clinically critical condition with a high mortality rate.Shenfu injection(SFI) is a Chinese herbal medicine extracted from red ginseng and Aconite,Radix Aconiti,with various pharmacological activities.This study aimed to investigate the potential mechanism of action of SFI in preventing sepsis-induced ALI.Materials and Methods:We established a mouse model of sepsis-induced ALI by cecal ligation and puncture(CLP).The mice were randomly divided into three groups(n=8):Sham,CLP,and SFI(10 mL/kg).Bronchoalveolar lavage fluid(BALF) and lung tissue were collected for pathological analysis,enzyme-linked immunosorbent assay,immunohistochemistry(IHC),and protein detection.Results:Our results showed that SFI significantly ameliorated pathological damage caused by CLP-induced ALI.SFI treatment significantly decreased the lung wet-to-dry weight ratio.In addition,SFI tr eatment significantly reduced the protein levels and cell numbers in the BALF SFI could significantly reduce the levels of tumor necrosis factor-α,interleukin-6(IL-6),and IL-1β in plasma and BALF.SFI significantly reduced the protein expression of B ax and cleaved caspase-3and increased the protein levels of Bcl-2.Western blotting and IHC results showed that SFI reduced the phosphorylation of Janus kinase2(JAK2) and signal transducer and activator of transcription 3(STAT3).Conclusions:In a septic ALI mouse model,SFI inhibited apoptosis and inflammation through the JAK2/STAT3 pathway,providing a candidate drug for the treatment of septic ALI.

关 键 词：Acute lung injury APOPTOSIS INFLAMMATION Janus kinase 2/signal transducer and activator of transcription 3 pathway Shenfu injection 

分 类 号：R285.5[医药卫生—中药学]