Effects of Regulating HSPA5 on Ferroptosis, Proliferation and Apoptosis of Cervical Cancer Cells in a Rat Model of Cervical Cancer  

作　　者：LIU Wen-fang 

机构地区：[1]Zhangzhou Maternal and Child Health Hospital,Zhangzhou Fujian province 363000,China

出　　处：《Chinese Journal of Biomedical Engineering(English Edition)》2024年第3期131-138,共8页中国生物医学工程学报(英文版)

摘　　要：Objective: To investigate the effects of regulating heat shock protein 70protein 5(HSPA5) on ferroptosis, proliferation and apoptosis of cervical cancer cells in a rat model of cervical cancer. Methods: A total of 48 SPF-grade healthy adult female Wistar rats were selected as the research objects. After adaptive feeding, U14 cells were intraperitoneally injected to construct a cervical cancer model. After successful modeling,the rats were randomly divided into four groups: the blank group was given normal saline by gavage for 7 d. The HM03+1-day group was given 10 mg/kg HM03 by gavage for 1 d followed by normal saline for 6 d, the HM03+3-day group was given 10 mg/kg HM03 by gavage for 3 d followed by normal saline for 4 d, and the HM03+7-day group was given10 mg/kg HM03 by gavage for 7 d. Cervical lesion tissues of the rats were taken to prepare cell suspensions, which were then cultured to assess the expressions of HSPA5,glutathione peroxidase 4(GPX4), overoxidized peroxidase 3(SO2/3-PRDX3), and iron(Fe^(2+))in the cervical cancer cells, as well as the proliferation and apoptosis of the cervical cancer cells, were detected. Subsequently, 6 cell suspensions from each group were treated with 10 μmol/L Ferrostatin-1(Fer-1) for 48 h, after which reactive oxygen species(ROS) and lactate dehydrogenase(LDH) expression were measured. Results: HSPA5 mRNA and protein expression in HM03-treated rat cervical cancer cells were significantly lower than those in the blank group(P<0.05), and with the extension of HM03 treatment time, HSPA5 mRNA and protein expression in rat cervical cancer cells were consequently lower(P<0.05). Relative expression of GPX4 protein in HM03-treated rat cervical cancer cells was significantly lower than that in the blank group(The relative expression of GPX4 protein in HM03-treated rat cervical cancer cells was significantly lower than that in the blank group(P<0.05), and the relative expression of SO_(2/3)-PRDX3protein and Fe^(2+)expression level were significantly higher than that in the blank g

关 键 词：cervical cancer HSPA5 ferroptosis cell proliferation APOPTOSIS 

分 类 号：R-332[医药卫生] R737.33