AFAP1-AS1在卵巢癌组织中的表达水平及其对卵巢癌生物学行为的影响  

The expression of AFAP1⁃AS1 in ovarian cancer tissue and its impact on the biological behavior of ovarian cancer

作　　者：江飞云 丁叶屏 刘星宇 蔡文 吴明彩[2] JIANG Fei-yun;DING Ye-ping;LIU Xing-yu;CAI Wen;WU Ming-cai(Department of Gynecology Wuhu Hospital of East China Normal University,Wuhu,Anhui Province 241399,China;Department of Microbiology,Wannan Medical College,Wuhu,Anhui Province 241001,China)

机构地区：[1]华东师范大学附属芜湖医院妇科,安徽芜湖241399 [2]皖南医学院微生物教研室,安徽芜湖241001

出　　处：《解剖学研究》2025年第1期43-50,共8页Anatomy Research

摘　　要：目的探讨肌动蛋白纤维相关蛋白1-反义RNA1(AFAP1-AS1)在卵巢癌组织中的表达以及其对卵巢癌细胞生物学行为的影响。方法采用RT-PCR检测73例卵巢癌患者的卵巢癌组织和癌旁组织中AFAP1-AS1的表达。对A2780卵巢癌细胞株进行体外培养,分为对照组(不转染)、si-NC组(转染si-NC)、siRNA组(转染小干扰RNA),MTT法检测3组细胞的增殖情况,检测细胞侵袭、迁移和上皮间质转化相关基因mRNA和蛋白的表达,F-actin染色检测细胞骨架排列情况。结果(1)卵巢癌、癌旁组织AFAP1-AS1表达分别为2.24±0.47、1.46±0.31,卵巢癌组织、癌旁组织AFAP1 mRNA、AFAP1蛋白分别为2.67±0.59、2.89±0.56和1.75±0.42、1.05±0.22,两者差异均有统计学意义(P<0.05);(2)对照组和si-NC组细胞增殖、转移、侵袭、EMT mRNA和蛋白表达以及骨架排列情况差异均无统计学意义(P>0.05);(3)培养48 h、72 h后siRNA组细胞吸光度值分别为0.65±0.16、0.88±0.22,均显著低于对照组1.04±0.20、1.59±0.26(P<0.05);(4)siRNA组迁移细胞数(167.62±29.88)、侵袭细胞数(148.43±33.64)和48 h迁移率(18.45±4.39)均显著低于对照组(438.76±48.92、467.50±53.27、46.87±5.63)(P<0.05);(5)si-RNA组AFAP1-AS1、N-cadherin、Vimentin表达降低,E-cadherin表达升高(P<0.05);(6)对照组和si-NC组细胞排列良好规则,在细胞边缘均可观察到大量的丝状伪足和大量肌动蛋白聚合。si-RNA组细胞排列紊乱,丝状伪足和激动蛋白聚合明显少于对照组,边缘呈现破损状。结论AFAP1-AS1高表达于卵巢癌中,下调AFAP1-AS1可以影响卵巢癌细胞上皮-间充质转化通路上蛋白的表达,抑制细胞的增殖、迁移和侵袭。Objective To investigate the expression of actinfilament⁃associated protein1⁃antisense RNA1(AFAP1⁃AS1)in ovarian cancer and its impact on the biological behavior of ovarian cancer.Methods RT⁃PCR was used to detect the expression of AFAP1⁃AS1 of 73 ovarian cancer patients.A2780 ovarian cancer cell line was cultured in vitro and divided into control group(non transfected),si⁃NC group(transfected with si⁃NC),and siR⁃NA group(transfected with small interfering RNA).MTT method was used to detect the proliferation,small cell ex⁃periments and scratch tests were used to detect cell invasion and migration,F⁃actin staining was used to detect the arrangement of cell bone shelves.The expression of epithelial mesenchymal transition(EMT)marker were detected.Results①The relative expression of AFAP1⁃AS1 in ovarian cancer tissue was 2.24±0.47,that of adjacent tissue was 1.46±0.31,with statistically significant differences(P<0.05).The mRNA and protein levels of AFAP1 in ovari⁃an cancer tissue were 2.67±0.59 and 2.89±0.56,respectively,while those in adjacent tissues were 1.75±0.42 and 1.05±0.22(P<0.05).②There was no statistically significant difference in cell proliferation,metastasis,invasion,EMT mRNA and protein expression,and skeletal arrangement between the control group and the si⁃NC group(P>0.05).③After 48 and 72 hours of cultivation,the proliferation of cells of the siRNA group(0.65±0.16,0.88±0.22)was significantly lower than control group(1.04±0.20,1.59±0.26)(P<0.05).④The migrated cells(167.62±29.88),invasive cells(148.43±33.64),and 48 hour migration rate(18.45±4.39)of the siRNA group were signifi⁃cantly lower than control group(438.76±48.92,467.50±53.27,46.87±5.63)(P<0.05).⑤The expressions of AFAP1⁃AS1,N⁃cadherin,and Vimentin mRNA/proteins of the si⁃RNA group were significantly lower,while the ex⁃pression of E⁃cadherin mRNA was significantly higher(P<0.05).⑥The cells in the control group and si⁃NC group were arranged well and regularly,with a large number

关 键 词：卵巢癌 肌动蛋白纤维相关蛋白1-反义RNA1 增殖 迁移 侵袭 长链非编码RNA 上皮-间充质转化 

分 类 号：R73[医药卫生—肿瘤]