MiR-340-5p affects the proliferation and transfer of gastric cancer cells by targeting lncRNA HCG11 and NRF2 expression  

作　　者：Rina Su Wei Luan Kai Zhang Shuai Liu Xiaoqing Zhang Yanwei Gao 

机构地区：[1]Abdominal Tumor Surgery,Inner Mongolia Autonomous Region People’s Hospital,Hohhot 010010,China [2]Department of Oncology,Inner Mongolia Autonomous Region People's Hospital,Hohhot 010010,China [3]Graduate School,Inner Mongolia Medical University,Hohhot 010059,China.

出　　处：《Oncology and Translational Medicine》2025年第1期29-35,共7页肿瘤学与转化医学(英文版)

基　　金：Funded by the general program of the Nature Fund of InnerMongolia Autonomous Region.

摘　　要：Objective:The aim of the study was to investigate the expression and biological role of nuclear factor erythroid–related factor 2(NRF2)in gastric cancer cells.Methods:Western blotting(WB)and reverse transcription-polymerase chain reaction(RT-PCR)were used to detect the expression of NRF2 in the tumor tissues of patients with gastric cancer and MKN45 cells.NRF2 was transfected into MKN45 gastric cancer cells,and apoptosis was detected using flow cytometry.The 3-(4,5-dimethylthiazole-2-yl)-2.5-dipheny-tetrazolium bromide(MTT)assay was per-formed to detect cell proliferation.Transwell and scratch experiments were performed to detect cell invasion and migration rates.WB blot-ting was performed to detect Bcl-2,COX-2,PEG2,Bax,and Cyt-C protein expression.RT-PCR was used to detect Bcl-2,COX-2,PEG2,Bax,Cyt-C,miR-340-5p,lncRNA HCG11,and NRF2 mRNA expression.Results:NRF2 expression increased in gastric cancer tissues and in the MKN45 gastric cancer cell model.Compared with the blank group,the shNRF2 knockdown group showed a significant increase in the MKN45 gastric cancer cell apoptosis rate(p<0.05)and a sig-nificant decrease in invasion and migration rates(p<0.05).WB and RT-PCR showed that,in the shNRF2 knockdown group,Bcl-2 protein and mRNA,miR-340-5p,and lncRNA HCG11 expression was significantly increased,whereas COX-2,PEG2,Bax,and Cyt-C protein and mRNA expression was significantly reduced(p<0.05).In the NRF2 overexpression group,Bcl-2 protein and mRNA expression was sig-nificantly reduced,whereas COX-2,PEG2,Bax,and Cyt-C protein and mRNA expression was significantly increased(p<0.05).The op-posite was observed for the NRF2 overexpression group.When miR-340-5p mimics were transfected into MKN45 gastric cancer cells,NRF2 and HCG11 expression significantly reduced(p<0.05).When the miR-340-5p inhibitor was transfected into MKN45 gastric cancer cells,NRF2 and HCG11 expression significantly increased(p<0.05).Conclusions:The invasion,migration,abnormal proliferation,and apoptosis of MKN45 gastric cancer cells are closely r

关 键 词：Gastric cancer Nuclear factor erythroid 2 related factor 2(NRF2) Apoptosis COX-2/PEG 2 signaling pathway 

分 类 号：R73[医药卫生—肿瘤]